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MilliporeSigma

A4656

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Mouse IgG (whole molecule)−AP

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1 ML

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$485.00


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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:

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Product Name

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat, affinity isolated antibody, buffered aqueous solution

biological source

goat

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

should not react with

human

technique(s)

direct ELISA: 1:1,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

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This Item
A3562A9316A5153
conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

technique(s)

direct ELISA: 1:1,000

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:7,000-1:21,000

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

species reactivity

mouse

species reactivity

mouse

species reactivity

-

species reactivity

-

biological source

goat

biological source

goat

biological source

goat

biological source

goat

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

Application

Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody has been used for ELISA assays.
Immunoprecipitated 293T cells lysates were analyzed by western blot using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary antibody at 1:7500.
Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at μg/ml in TBS/Tween containing final concentration of 0.5M NaCl.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody binds to mouse IgGs and does not cross-react with human serum proteins.

Immunogen

purified mouse IgG

Other Notes

Antibody adsorbed with human serum proteins.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background with human samples.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Carlos Andrés Rodriguez-Salazar et al.
Viruses, 14(2) (2022-02-27)
Dengue virus is a ssRNA+ flavivirus, which produces the dengue disease in humans. Currently, no specific treatment exists. siRNAs regulate gene expression and have been used systematically to silence viral genomes; however, they require controlled release. Liposomes show favorable results
P J Simpson et al.
Circulation, 81(1), 226-237 (1990-01-01)
Pentobarbital anesthetized dogs were subjected to 90 minutes of left circumflex coronary artery (LCCA) occlusion followed by 72 hours of reperfusion. Control or anti-Mo1 (904) F(ab')2 fragments of monoclonal antibodies were administered intravenously at a dose of 1 mg/kg beginning
Delia Piedad Recalde-Reyes et al.
Peptides, 153, 170797-170797 (2022-04-05)
Dengue virus (DENV) infection is mediated by the interaction between the virus envelope protein and cellular receptors of the host cells. In this study, we designed peptides to inhibit protein-protein interaction between dengue virus and CD44 receptor, which is one
The LIM-only Protein DRAL/FHL2 Interacts with and Is a Corepressor for the Promyelocytic Leukemia Zinc Finger Protein
McLoughlin, P.
The Journal of Cell Biology, 277, 37045-37053 (2002)
Clara E Green et al.
Biomedicines, 9(7) (2021-08-07)
The pulmonary endothelium is dysfunctional in chronic obstructive pulmonary disease (COPD), a known risk factor for lung cancer. The pulmonary endothelium is altered in emphysema, which is disproportionately affected by cancers. Gene and microRNA expression differs between COPD and non-COPD

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