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A4656

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Goat Anti-Mouse IgG (whole molecule)−AP
MDL number:
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

should not react with

human

technique(s)

direct ELISA: 1:1,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
A3688A3562A5153
biological source

goat

biological source

goat

biological source

goat

biological source

goat

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

-

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody binds to mouse IgGs and does not cross-react with human serum proteins.

Immunogen

purified mouse IgG

Application

Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody has been used for ELISA assays.
Immunoprecipitated 293T cells lysates were analyzed by western blot using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary antibody at 1:7500.
Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at μg/ml in TBS/Tween containing final concentration of 0.5M NaCl.

Other Notes

Antibody adsorbed with human serum proteins.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background with human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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