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Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal

Name: Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal
Brand: SIGMA
Price: 363 USD
Availability: InStock

clone RG-96, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Rabbit IgG (γ-chain specific)

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Size/SKU	Availability	Price
1 vial	Check Cart for Availability	$363.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

peroxidase conjugate

Clone:

RG-96, monoclonal

Application:

ELISA (d), IHC (p), WB

Citations:

$363.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

RG-96, monoclonal

form

lyophilized powder

species reactivity

rabbit

should not react with

goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken

packaging

vial of 0.5 mL

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well)

isotype

IgG1

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Anti-Rabbit IgG (γ-chain specific)-Peroxidase antibody, Mouse monoclonal is a monoclonal anti-rabbit IgG conjugated to horseradish peroxidase. Monoclonal antirabbit IgG is derived from the RG-96 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG.

Rabbit IgGs are often used as primary antibodies in several immunological assays. Thus, polyclonal anti-rabbit IgGs are usually used as secondary antibodies in such applications. However, polyclonal antibodies to rabbit IgGs can also cross-react with IgGs from other species, especially with human IgGs, and thereby lead to a lack of specificity in test results. The use of monoclonal anti-rabbit IgG that is specific for only rabbit IgG improves the accuracy of immunological assays and thus, can be useful for various research applications.

The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.

Immunogen

Rabbit IgG

Application

Monoclonal Anti-Rabbit IgG (γ-chain specific)- Peroxidase has been used in

various immunochemical assays like enzyme linked immunosorbent assay (ELISA)
westernblotting
immunohistology
dot immunobinding assay

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A3673	A9452	A0168
Sigma-Aldrich A1949 Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal	Sigma-Aldrich A3673 Anti-Mouse IgG (γ-chain specific)−Peroxidase antibody produced in goat	Sigma-Aldrich A9452 Anti-Goat/Sheep IgG−Peroxidase antibody, Mouse monoclonal	Sigma-Aldrich A0168 Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat
description clone RG-96, purified from hybridoma cell culture	description affinity isolated antibody, buffered aqueous solution	description clone GT-34, purified from hybridoma cell culture	description affinity isolated antibody
technique(s) direct ELISA: 1:40,000, western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200	technique(s) direct ELISA: 1:15,000	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, dot blot: 1:160,000 (chemiluminescent), direct ELISA: 1:30,000	technique(s) western blot: 1:80,000-1:160,000 using total cell extract of HeLa cells (5-10 μg per well, direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, immunocytochemistry: suitable
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate
antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody
species reactivity rabbit	species reactivity mouse	species reactivity sheep, goat, bovine	species reactivity mouse
biological source mouse	biological source goat	biological source mouse	biological source goat
isotype IgG1	isotype -	isotype IgG1	isotype -

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Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H317

Precautionary Statements

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

13 - Non Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)