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A1949

Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal

Name: Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal
Brand: SIGMA
Price: 1846 BRL

clone RG-96, purified from hybridoma cell culture

Sinônimo(s):

Monoclonal Anti-Rabbit IgG (γ-chain specific)

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Para você/SKU	Disponibilidade	Preço
1 vial	Verificar disponibilidade no carrinho	R$ 1.846,00

Sobre este item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

peroxidase conjugate

Clone:

RG-96, monoclonal

Application:

ELISA (d), IHC (p), WB

Citations:

R$ 1.846,00

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Propriedades

biological source

mouse

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

RG-96, monoclonal

form

lyophilized powder

species reactivity

rabbit

should not react with

goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken

packaging

vial of 0.5 mL

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well)

isotype

IgG1

storage temp.

2-8°C

target post-translational modification

unmodified

Descrição

General description

Anti-Rabbit IgG (γ-chain specific)-Peroxidase antibody, Mouse monoclonal is a monoclonal anti-rabbit IgG conjugated to horseradish peroxidase. Monoclonal antirabbit IgG is derived from the RG-96 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG.

Rabbit IgGs are often used as primary antibodies in several immunological assays. Thus, polyclonal anti-rabbit IgGs are usually used as secondary antibodies in such applications. However, polyclonal antibodies to rabbit IgGs can also cross-react with IgGs from other species, especially with human IgGs, and thereby lead to a lack of specificity in test results. The use of monoclonal anti-rabbit IgG that is specific for only rabbit IgG improves the accuracy of immunological assays and thus, can be useful for various research applications.

The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.

Immunogen

Rabbit IgG

Application

Monoclonal Anti-Rabbit IgG (γ-chain specific)- Peroxidase has been used in

various immunochemical assays like enzyme linked immunosorbent assay (ELISA)
westernblotting
immunohistology
dot immunobinding assay

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Este Item	A2074	A2556	R3155
Sigma-Aldrich A1949 Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal	Sigma-Aldrich A2074 Monoclonal Anti-Rabbit Immunoglobulins–Peroxidase antibody produced in mouse	Sigma-Aldrich A2556 Monoclonal Anti-Rabbit IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in mouse	Sigma-Aldrich R3155 Anti-Rabbit IgG, Native−Peroxidase antibody, Mouse monoclonal
description clone RG-96, purified from hybridoma cell culture	description clone RG-16, purified immunoglobulin, lyophilized powder	description clone RG-96, purified immunoglobulin, buffered aqueous glycerol solution	description clone RabT-50, purified from hybridoma cell culture
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate alkaline phosphatase conjugate	conjugate peroxidase conjugate
biological source mouse	biological source mouse	biological source mouse	biological source mouse
species reactivity rabbit	species reactivity rabbit	species reactivity rabbit	species reactivity rabbit
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin
technique(s) direct ELISA: 1:40,000, western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200	technique(s) direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissue, western blot (chemiluminescent): 1:160,000	technique(s) direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells	technique(s) immunocytochemistry: suitable, indirect ELISA: suitable, western blot: 1:10,000-1:20,000 using 3T3 cell extracts using Anti-Actin (20-33), Catalog Number A5060, as the primary antibody, western blot: 1:10,000-1:20,000 using Hela cell extracts using Anti-Actin, N-terminal, Catalog Number A2103, as the primary antibody.
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C

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pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Classe de armazenamento

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c