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Phosphatase, Alkaline shrimp

Name: Phosphatase, Alkaline shrimp
Brand: SIGMA

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

Synonym(s):

Phosphatase, Alkaline from shrimp, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

CAS Number:

9001-78-9

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.1.3.1 (BRENDA, IUBMB)

MDL number:

MFCD00131849

eCl@ss:

42010105

Recombinant:

expressed in proprietary host

Concentration:

≥900 DEA units/mL

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Properties

recombinant

expressed in proprietary host

Quality Segment

200

form

buffered aqueous glycerol solution

concentration

≥900 DEA units/mL

foreign activity

RNAse, DNAse, none detected

shipped in

dry ice

storage temp.

−20°C

Description

General description

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.

Application

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice.[1] It also may be used in pasteurization process for milks used in dairy products.[2]

Biochem/physiol Actions

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.

Physical form

solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl₂

Analysis Note

Protein determined by biuret

Other Notes

One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.

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This Item	P5931	P6774	P5521
Sigma-Aldrich A2237 Phosphatase, Alkaline shrimp	Sigma-Aldrich P5931 Phosphatase, Alkaline from Escherichia coli	Sigma-Aldrich P6774 Phosphatase, Alkaline from bovine intestinal mucosa	Sigma-Aldrich P5521 Phosphatase, Alkaline from bovine intestinal mucosa
description ≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host	description lyophilized powder, 30-60 units/mg protein (in glycine buffer)	description buffered aqueous solution, ≥2,000 DEA units/mg protein	description ≥2,000 DEA units/mg protein
form buffered aqueous glycerol solution	form lyophilized powder	form buffered aqueous solution	form aqueous solution
recombinant expressed in proprietary host	recombinant -	recombinant -	recombinant -
concentration ≥900 DEA units/mL	concentration -	concentration 5-20 mg/mL	concentration ≥1.0 mg/mL
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in dry ice	shipped in -	shipped in -	shipped in -
foreign activity RNAse, DNAse, none detected	foreign activity -	foreign activity -	foreign activity -

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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