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A2237

Phosphatase, Alkaline shrimp

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

Synonym(s):

Phosphatase, Alkaline from shrimp, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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Size/SKUAvailabilityPrice
500 units
Please contact Customer Service for Availability
$301.00
1000 units
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$553.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
eCl@ss:
42010105
Recombinant:
expressed in proprietary host
Concentration:
≥900 DEA units/mL

$301.00


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recombinant

expressed in proprietary host

Quality Segment

form

buffered aqueous glycerol solution

concentration

≥900 DEA units/mL

technique(s)

protein detection: suitable

foreign activity

RNAse, DNAse, none detected

shipped in

dry ice

storage temp.

−20°C

General description

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.

Application

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice. It also may be used in pasteurization process for milks used in dairy products.

Biochem/physiol Actions

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.

Physical form

solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl2

Analysis Note

Protein determined by biuret

Other Notes

One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.

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This Item
GEE70092YGEE70092XP0114
description

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

description

Cytiva E70092Y, pack of 500 U

description

Cytiva E70092X, pack of 5000 U

description

BioUltra, ≥5,700 DEA units/mg protein

form

buffered aqueous glycerol solution

form

liquid

form

-

form

(40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

recombinant

expressed in proprietary host

recombinant

-

recombinant

-

recombinant

-

concentration

≥900 DEA units/mL

concentration

-

concentration

-

concentration

≥10 mg/mL

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

foreign activity

RNAse, DNAse, none detected

foreign activity

-

foreign activity

-

foreign activity

-


Pictograms

Health hazard

Signal Word

Danger

Hazard Codes

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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