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A2237

Phosphatase, Alkaline shrimp

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

Synonyme(s) :

Phosphatase, Alkaline from shrimp, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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500 units
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282.00 CHF
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470.00 CHF

A propos de cet article

Numéro CAS:
9001-78-9
UNSPSC Code:
12352204
NACRES:
NA.54
Numéro CE :
3.1.3.1 (BRENDA, IUBMB)
MDL number:
MFCD00131849
eCl@ss:
42010105
Recombinant:
expressed in proprietary host
Concentration:
≥900 DEA units/mL

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recombinant

expressed in proprietary host

Quality Segment

200

form

buffered aqueous glycerol solution

concentration

≥900 DEA units/mL

foreign activity

RNAse, DNAse, none detected

shipped in

dry ice

storage temp.

−20°C

General description

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.

Application

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice.[1] It also may be used in pasteurization process for milks used in dairy products.[2]

Biochem/physiol Actions

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.

Physical form

solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl2

Analysis Note

Protein determined by biuret

Other Notes

One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.

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Cet article
P7923P5931P6774
Phosphatase, Alkaline shrimp ≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

Sigma-Aldrich

A2237

Phosphatase, Alkaline shrimp

Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous glycerol solution, ≥4,000 DEA units/mg protein

Sigma-Aldrich

P7923

Phosphatase, Alkaline from bovine intestinal mucosa

Phosphatase, Alkaline from Escherichia coli lyophilized powder, 30-60 units/mg protein (in glycine buffer)

Sigma-Aldrich

P5931

Phosphatase, Alkaline from Escherichia coli

Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous solution, ≥2,000 DEA units/mg protein

Sigma-Aldrich

P6774

Phosphatase, Alkaline from bovine intestinal mucosa

description

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

description

buffered aqueous glycerol solution, ≥4,000 DEA units/mg protein

description

lyophilized powder, 30-60 units/mg protein (in glycine buffer)

description

buffered aqueous solution, ≥2,000 DEA units/mg protein

recombinant

expressed in proprietary host

recombinant

-

recombinant

-

recombinant

-

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

lyophilized powder

form

buffered aqueous solution

concentration

≥900 DEA units/mL

concentration

≥10.0 mg/mL

concentration

-

concentration

5-20 mg/mL

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

foreign activity

RNAse, DNAse, none detected

foreign activity

-

foreign activity

-

foreign activity

-

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Classe de stockage

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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