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A2289

Albumin, Tetramethylrhodamine isothiocyanate bovine

lyophilized powder

Synonym(s):

TRITC-albumin

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10 mg
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$486.00
50 mg
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$1,860.00

About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.25
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥90% (Lowry)
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
Impurities:
salt, free
Concentration:
≥90% protein (Lowry)

$486.00


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biological source

bovine

Quality Level

assay

≥90% (Lowry)

form

lyophilized powder

concentration

≥90% protein (Lowry)

extent of labeling

≥0.5 mol TRITC per mol albumin

technique(s)

cell culture | mammalian: suitable

impurities

salt, free

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

General description

Tetramethylrhodamine isothiocyanate (TRITC) is stable and less sensitive to light compared to fluorescein isothiocyanate (FITC). The conjugate of albumin with TRITC is effective for following microcirculation in vivo microscopy. Albumin-TRITC aids tracking of calcium phosphate nanoparticle bound DNA in human umbilical vein endothelial cells (T-HUVEC).

Application

Red-fluorescing TRITC-albumin was used to track the uptake of calcium phosphate/DNA nanoparticles during cell transfection. The dye-linked albumin is incorporated into the nanoparticles. Efficiency of transfection could be followed by fluorescence microscopy.
Albumin, Tetramethylrhodamine isothiocyanate has been used:
  • in photoinduced release assay of polyethylene beads
  • for passivating poly(methyl methacrylate) (PMMA) layer prior to immunoglobulin G coating
  • in fluorescent correlation spectroscopy

Preparation Note

Prepared from crystallized and lyophilized albumin (A4378).

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This Item
N8159A3264A8426
technique(s)

cell culture | mammalian: suitable

technique(s)

immunoblotting: suitable

technique(s)

hybridization: suitable, microbiological culture: suitable, protein quantification: suitable

technique(s)

cell culture | mammalian: suitable

biological source

bovine

biological source

bovine

biological source

sheep

biological source

bovine

assay

≥90% (Lowry)

assay

-

assay

≥97% (agarose gel electrophoresis)

assay

≥90% protein basis (biuret)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

Gene Information

bovine ... ALB(280717)

Gene Information

bovine ... ALB(280717)

Gene Information

sheep ... ALB(443393)

Gene Information

bovine ... ALB(280717)


pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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Tracking the pathway of calcium phosphate/DNA nanoparticles during cell transfection by incorporation of red-fluorescing tetramethylrhodamine isothiocyanate-bovine serum albumin into these nanoparticles
Sokolova V, et al.
Journal of Biological Inorganic Chemistry, 12(2), 174-179 (2007)
Photoresponsive Aqueous Dissolution of Poly (N-Isopropylacrylamide) Functionalized with o-Nitrobenzaldehyde through Phase Transition
Sumaru K, et al.
Biomacromolecules, 19(7), 2913?2922-2913?2922 (2018)
Fluorescent Biorecognition of Gold Nanoparticle-IgG Conjugates Self-Assembled on E-Beam Patterns
Powell T and Yoon JY
Biotechnology Progress, 22(1), 106-110 (2006)



Global Trade Item Number

SKUGTIN
A2289-10MG04061833267196
A2289-5MG04061831151916
A2289-50MG04061833267202

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