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Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat

Name: Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat
Brand: SIGMA
Price: 320 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Mouse IgG (Fab specific)- HRP

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Size/SKU	Availability	Price
1 mL	Available to ship TODAYfromMILWAUKEE	$320.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162623

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

ELISA (d), IHC (p), WB

Citations:

153

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Properties

biological source

goat

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

should not react with

bovine, horse, human

technique(s)

direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue section, western blot: 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense and is a glycoprotein. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat anti-mouse IgG (Fab specific)-peroxidase antibody associates with mouse IgG and mouse Fab fragment. The conjugate shows no reactivity with mouse Fc fragment, human IgG, IgA, IgM, kappa and lambda light chain, bovine IgG and IgM, or horse IgG.

Application

Anti-Mouse IgG (Fab specific)–Peroxidase antibody has been used in western blotting.

Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody.

Goat anti-mouse IgG (Fab specific)-peroxidase antibody is suitable for use in immunohistochemistry applications at 1:200 dilution using human tonsil tissues. The antibody can also be used for western blot assays at 1:80,000-1:160,000 dilutions using total cell extract of HeLa cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Preparation Note

Adsorbed to reduce background staining with bovine, horse and human samples.

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Other Notes

Antibody adsorbed with bovine, equine, and human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A9309	A9917	A3682
Sigma-Aldrich A2304 Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat	Sigma-Aldrich A9309 Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat	Sigma-Aldrich A9917 Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat	Sigma-Aldrich A3682 Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution
species reactivity mouse	species reactivity -	species reactivity mouse	species reactivity mouse
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
biological source goat	biological source goat	biological source goat	biological source goat
technique(s) direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue section, western blot: 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells	technique(s) western blot: 1:40,000-1:80,000 using total cell extract of HeLa cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, direct ELISA: 1:40,000	technique(s) western blot: 1:80,000-1:160,000, direct ELISA: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150, western blot: 1:80,000-160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per lane), direct ELISA: 1:40,000
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

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Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H317

Precautionary Statements

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

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