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Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat

Name: Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat
Brand: SIGMA
Price: 254 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Mouse IgG (Fc specific)–AP

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Size/SKU	Availability	Price
0.5 mL	Estimated to ship onNovember 09, 2026fromMILWAUKEE	$254.00
1 mL	Estimated to ship onNovember 09, 2026fromMILWAUKEE	$430.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162626

Conjugate:

alkaline phosphatase conjugate

Clone:

polyclonal

Application:

ELISA (d), IHC (p), WB CL

Citations:

$254.00

Estimated to ship onNovember 09, 2026Details

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Properties

biological source

goat

Quality Segment

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

should not react with

bovine, human, horse

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40, western blot (chemiluminescent): 1:160,000-1:320,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG1 regulates complement fixation in mice.

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.

Immunogen

Purified mouse IgG, Fc fragment

Application

Alkaline phosphatase-conjugated goat anti-mouse Fc specific antibody was used as a secondary antibody in ELISA assays at a dilution of 1:1000 in PBS/0.1% Tween and 1% BSA for 1.5 hours at 37°C. Antibody was developed using 4-nitrophenyl phosphate (Sigma) as a substrate for 30 minutes at 37°C.

Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is suitable for use in ELISA (1:1500) and western blot. The antibody can also be used for immunohistochemistry (1:40 using formalin-fixed, paraffin-embedded sections).

Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA), sandwich ELISA and enzyme-linked immune absorbent spot (ELISPOT).

The product has been used as a secondary antibody for detecting mouse serum anti-hemagglutinin (HA) antibodies using ELISA techniques.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with bovine, horse, or human samples.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A1682	A2179	A3438
Sigma-Aldrich A2429 Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A1682 Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A2179 Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A3438 Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous glycerol solution
biological source goat	biological source goat	biological source goat	biological source goat
species reactivity mouse	species reactivity mouse	species reactivity mouse	species reactivity mouse
conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
technique(s) western blot (chemiluminescent): 1:160,000-1:320,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40, direct ELISA: 1:30,000	technique(s) western blot: 1:200,000-1:400.000 using assay detecting β-Actin in total cell extract of HeLa cells (5-10 μg per well), direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10	technique(s) direct ELISA: 1:40,000, western blot (chemiluminescent): 1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25	technique(s) direct ELISA: 1:30,000, dot blot: 1:30,000, western blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous glycerol solution

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Storage Class

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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