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A25120

DEAE–Sephadex® A-25 chloride form

Synonym(s):

DEAE Sephadex®, Sephadex A 25,2-(diethylamino)ethylether, Diethylaminoethyl Sephadex®

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Size/SKUAvailabilityPrice
10 g

Available to ship TODAYfromMILWAUKEE

$146.00
50 g

Available to ship TODAYfromMILWAUKEE

$635.00

About This Item

CAS Number:
12609-80-2
UNSPSC Code:
47101511
NACRES:
NA.56
MDL number:
MFCD00132778

$146.00

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form

powder

Quality Segment

technique(s)

activity assay: suitable

matrix

cross-linked dextran

matrix active group

functional group

bead size

40-125 μm (dry)

pore size

~30,000 Da exclusion limit

operating pH

2-9

capacity

3-4 meq/g ion exchange capacity

compatibility

mode of use mixed weak and strong anion exchange chromatography

General description

The Sephadex® ion exchangers contain a cross-linked dextran matrix with functional groups. These groups are linked by stable ether linkages to the glucose units in the matrix. DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins.

A25120-100G′s updated product number is GE17-0170-01
A25120-500G′s updated product number is GE17-0180-02

Application

DEAE-Sephadex® A-25 has been used:
  • to form DEAE-Sephadex A-25 (formate form) for glucosinolates (GLS) extraction using horseradish plants
  • in the estimation of GLS content in Chinese kale sprouts
  • production and purification of endozepine from astrocytes
  • to identify and label an insulin-activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients

Features and Benefits

  • Weak anion exchanger
  • Suitable for batch techniques
  • High binding capacities
  • Non-reactive to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride

Legal Information

DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

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This Item
Q25120A50120C50120
DEAE–Sephadex® A-25 chloride form

Sigma-Aldrich

A25120

DEAE–Sephadex® A-25 chloride form

QAE Sephadex® A-25 chloride form

Sigma-Aldrich

Q25120

QAE Sephadex® A-25 chloride form

DEAE–Sephadex®

Sigma-Aldrich

A50120

DEAE–Sephadex®

CM Sephadex® C-50

Sigma-Aldrich

C50120

CM Sephadex® C-50

description

-

description

-

description

-

description

-

matrix active group

functional group

matrix active group

-

matrix active group

-

matrix active group

-

matrix

cross-linked dextran

matrix

-

matrix

cross-linked dextran

matrix

-

pore size

~30,000 Da exclusion limit

pore size

~30,000 Da exclusion limit

pore size

~200,000 Da exclusion limit

pore size

~200,000 Da exclusion limit

operating pH

2-9

operating pH

2-10

operating pH

-

operating pH

6-10

form

powder

form

-

form

powder

form

powder

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

LPLC: suitable, protein purification: suitable

technique(s)

activity assay: suitable

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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Questions

1–5 of 5 Questions  

  1. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  2. What is the chemical stability of Product A25120, DEAE-Sephadex® A-25 chloride form?

    1 answer

    1. According to the manufacturer, this is compatible with all commonly used aqueous buffers, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride and 70% ethanol.

      Helpful?

  3. How should I regenerate Product A25120, DEAE-Sephadex® A-25 chloride form?

    1 answer

    1. After each separation, elute any reversibly bound material either with a high ionic strength solution (e.g. 1 M NaCl in buffer) or by increasing pH. Regenerate the media by washing with at least 5 bed volumes of buffer, or until the column effluent shows stable conductivity and pH values.

      Helpful?

  4. How should I store Product A25120, DEAE-Sephadex® A-25 chloride?

    1 answer

    1. Unused media can be stored in the container at +4 to +30 °C.  Ensure that the screw-top is fully tightened.  Packed columns should be equilibrated in working buffer containing 20% ethanol to prevent microbial growth.

      Helpful?

  5. How do I equilibrate Product A25120, DEAE-Sephadex® A-25 chloride form?

    1 answer

    1. After packing, and before a chromatographic run, equilibrate with working buffer by washing with at least 5 bed volumes.

      Helpful?

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