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A3151

Avidin–Peroxidase

lyophilized powder

Synonym(s):

Avidin-POD

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1 mg
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$152.00
2 mg
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$259.00
5 mg

Available to ship TODAYfromMILWAUKEE

$527.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00130587
Form:
lyophilized powder
Biological source:
avidin from egg white, enzyme from horseradish

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Quality Level

200

biological source

avidin from egg white, enzyme from horseradish

conjugate

peroxidase conjugate

form

lyophilized powder

composition

Protein, ≥70% E1%/280

extent of labeling

0.7-2.0 mol peroxidase per mol avidin

technique(s)

direct ELISA: 1:50,000

solubility

H2O: soluble 1 mg/mL, PBS: soluble

storage temp.

−20°C

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues[1]. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin-Peroxidase has been used for detecting anti-5-bromodeoxyuridine (anti-BrdU) binding[2] and OVA-IgG1[3].
To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees.

Physical form

Lyophilized powder containing citrate buffer.

Preparation Note

Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
A7294A7419A2901
Avidin–Peroxidase lyophilized powder

Sigma-Aldrich

A3151

Avidin–Peroxidase

Avidin–Alkaline Phosphatase buffered aqueous solution

Sigma-Aldrich

A7294

Avidin–Alkaline Phosphatase

Avidin–Peroxidase buffered aqueous solution

Sigma-Aldrich

A7419

Avidin–Peroxidase

Avidin–FITC from egg white lyophilized powder

Sigma-Aldrich

A2901

Avidin–FITC from egg white

technique(s)

direct ELISA: 1:50,000

technique(s)

direct ELISA: 1:70,000, western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane

technique(s)

indirect ELISA: 1:100,000, western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well)

technique(s)

immunohistochemistry: suitable

biological source

avidin from egg white, enzyme from horseradish

biological source

avidin from egg white, enzyme from bovine (calf) intestine

biological source

avidin from egg white, enzyme from horseradish

biological source

-

form

lyophilized powder

form

buffered aqueous solution

form

buffered aqueous solution

form

lyophilized powder

solubility

H2O: soluble 1 mg/mL, PBS: soluble

solubility

-

solubility

-

solubility

-

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

peroxidase conjugate

conjugate

FITC conjugate

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCM5375
SLCD1995
SLCB6957
SLBW7331
SLBV8230

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Global Trade Item Number

SKUGTIN
A3151-1MG04061835540570
A3151-2MG04061833360408
A3151-5MG04061833360415

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