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A3437

Anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Goat Anti-Human IgM (μ-chain specific)−AP

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0.25 mL

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€113.00
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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162460
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p), WB
Citations:
20

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biological source

goat

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Human IgMs are the initial immunoglobulin isotypes that appear in blood in response to the first exposure to external antigens. These immunoglobulins have been implicated in CNS myelin repair. Increased levels of secretory IgM have been linked to rhino-conjunctivitis . Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for human IgM when tested against human IgA, IgG, IgM, Bence Jones κ, and λ myeloma proteins.
Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection.
Alkaline Phosphatase is an enzyme that catalyzes the conversion of chromogenic substrates such as p-nitrophenylphosphate (PNPP); chemiluminescent substrates such as CDP-Star® and fluorogenic substrates such as 4-methylumbelliferyl phosphate (4-MUP) into detectable chromophores, light-emitters or fluorescers, respectively.

Immunogen

Human IgM

Application

Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is suitable for use in ELISA.
Goat Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody can be used for dot blot and western blot applications at 1:30,000.
Goat polyclonal anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody may be used to detect and quantitate the level of IgM in human serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques.
IgM is a glycoprotein with 5 n-linked glycosylation sites on the heavy chain. An ELISA assay was performed to identify glycosylated forms of IgM that bind to lectin. Alkaline phosphatase conjugated goat anti-human IgM was used as the secondary at 1:2000 and developed using p-nitrophenyl substrate (Sigma).
IgM is a glycoprotein with 5 n-linked glycosylation sites on the heavy chain. An ELISA assay was performed to identify glycosylated forms of IgM that bind to lectin. Alkaline phosphatase conjugated goat anti-human IgM was used as the secondary at 1:2000 and developed using p-nitrophenyl substrate (Sigma). Arnold, J. (2005) Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin. JBC, 280: 29080-29087.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Legal Information

CDP-Star is a registered trademark of Tropix, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3437

Anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody produced in goat

Anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

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Anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody produced in goat

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Sigma-Aldrich

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Anti-Human IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3187

Anti-Human IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

goat

biological source

goat

biological source

goat

biological source

goat

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000, dot blot: 1:30,000

technique(s)

direct ELISA: 1:50,000, dot blot: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot (chemiluminescent): 1:60,000

technique(s)

direct ELISA: 1:7,000-1:21,000

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

species reactivity

human

species reactivity

-

species reactivity

human

species reactivity

human

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000545849
0000545849
0000500144
0000500151
0000500150

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Related Content

Data Sheet - A3437

Instructions

F Petry
Infection, 26(1), 7-10 (1998-03-20)
In a seroprevalence study including 495 sera from persons of all age-groups, the presence of anti-Cryptosporidium parvum antibodies was evaluated in an enzyme immunoassay. Despite the fact that C. parvum is only found in approximately 2% of patients with diarrhea
Helena Cerutti et al.
Journal of clinical laboratory analysis, 35(4), e23735-e23735 (2021-02-21)
The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has generated a pandemic with alarming rates of fatality worldwide. This situation has had a major impact on clinical laboratories that have attempted to answer the urgent need for
Chunsheng Jin et al.
The Journal of biological chemistry, 298(4), 101732-101732 (2022-02-18)
Changes in glycosphingolipid structures have been shown to occur during the development of several types of human cancers, generating cancer-specific carbohydrate structures that could be used as biomarkers for diagnosis and therapeutic targeting. In this study, we characterized nonacid glycosphingolipids
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Global Trade Item Number

SKUGTIN
A3437-.25ML04061838352170
A3437-.5ML04061838352187
A3437-1ML04061833360927

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