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A3525

Anti-Human IgE (ε-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Goat Anti-Human IgE (ε-chain specific)−AP

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0.5 mL
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$375.00
1 mL
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$643.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162428
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
23

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biological source

goat

Quality Segment

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

direct ELISA: 1:7,000-1:21,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Affinity isolated antibody is obtained from anti-human IgE antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the ε-chain of human IgE. Anti-Human IgE is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde. IgE is present in low levels compared to other immunoglobulin isotypes. It exists in two forms, one being a secreted by plasma cell and the other form is associated with the B cell receptor.
IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma . Anti-Human IgE (epsilon-chain specific)-Alkaline Phosphatase antibody is specific for human IgE when tested against human IgA, IgG, IgM, Bence Jones κ and λ myeloma proteins.

Immunogen

Purified human IgE

Application

Anti-Human IgE (ε-chain specific)−Alkaline Phosphatase antibody produced in goat has been used in dot blot.
Anti-Human IgE (epsilon-chain specific)-Alkaline Phosphatase antibody is suitable for use in immunoblotting and ELISA (at 1:500 dilutions using 100μ/well).
IgE secreted from transfected murin myeloma cells was detected by ELISA using the alkaline phosphatase-conjugated goat anti-human IgE (epsilon specific) antibody.

Biochem/physiol Actions

IgE bound to B cell receptor mediates antigen uptake and regulates differentiation and proliferation of B cells.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 15 mM sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Human IgE (ε-chain specific)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

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Anti-Human IgA (α-chain specific)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

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Anti-Human IgA (α-chain specific)−Alkaline Phosphatase antibody produced in goat

description

affinity isolated antibody, buffered aqueous glycerol solution

description

affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous glycerol solution

description

affinity isolated antibody, buffered aqueous solution

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

-

biological source

goat

biological source

goat

biological source

goat

biological source

goat

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

technique(s)

direct ELISA: 1:7,000-1:21,000

technique(s)

direct ELISA: 1:7,000-1:21,000

technique(s)

direct ELISA: 1:7,000-1:21,000

technique(s)

direct ELISA: 1:35,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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