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A3563

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Alkaline Phosphatase antibody produced in sheep

affinity isolated antibody, buffered aqueous glycerol solution

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A voi/SKUDisponibilitàPrezzo
0.5 mL
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
212,00 €
1 mL
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
367,00 €

Informazioni su questo articolo

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162640
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p), WB
Citations:
9

212,00 €

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biological source

sheep

Quality Segment

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

mouse

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice
Sheep anti-mouse IgG (whole molecule) F(ab′)2 fragment is specific for the F(ab′)2 of mouse IgG. The antibody preparation consists only of the F(ab′)2 fragment of sheep IgG as determined by SDS-PAGE. No contamination with sheep IgG whole molecule is observed.

Immunogen

Mouse IgG

Application

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:30,000), western blot (1:30,000), and immunohistochemistry (at 1:50 dilution in formalin-fixed, paraffin-embedded sections). The antibody may also be used for dot blot and immunoblot assays using a working dilution of 1:30,000.
ELISAs using alkaline phosphatase conjugated sheep anti-mouse IgG whole molecule antibody were performed to measure the antibody response in the sera of mice immunixed to T. crassiceps. 1:1000 at 37 degrees for one hour and developed using p-nitrophenyl (Sigma).

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Questo articolo
A7434A3438A1293
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Alkaline Phosphatase antibody produced in sheep affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3563

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Alkaline Phosphatase antibody produced in sheep

Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A7434

Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat

Anti-IgG di topo (specifico per catena γ)−fosfatasi alcalina affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3438

Anti-IgG di topo (specifico per catena γ)−fosfatasi alcalina

Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A1293

Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat

biological source

sheep

biological source

goat

biological source

goat

biological source

goat

species reactivity

mouse

species reactivity

-

species reactivity

mouse

species reactivity

mouse

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, dot blot: 1:30,000, western blot: 1:30,000

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, western blot (chemiluminescent): 1:30,000 using β-actin in total cell extract of HiLa cells (5-10 μg per well)

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot (chemiluminescent): 1:30,000

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

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Classe di stoccaggio

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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