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A3687

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Alkaline Phosphatase Secondary Antibody, Anti Rabbit Antibody - Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat, Anti Rabbit Sigma, anti rabbit antibody

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0.25 mL
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$154.00
0.5 mL
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$270.00
1 mL
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$457.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162782
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB
Citations:
339

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biological source

goat

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

rabbit

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody binds all rabbit Igs.

Immunogen

purified rabbit IgG as the immunogen

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Dot blot (1 paper)
Enzyme-linked immunosorbent assay (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (10 papers)
Citrullination of antithrombin by PADI4 was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary diluted at 1:5000 in 0.05M carbonate/bicarbonate buffer (Ph 9.6).
Goat anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody has been used for western blot and ELISA applications.the antibody can also be used for immunohistochemistry (at 1:50, using formalin-fixed, paraffin-embedded sections).
The level of hypoxyprobe binding proteins present in retinal mouse tissue was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary at a dilution of 1:2000 in 5% tween/PBS. Substrate used was 4-nitrophenyl phosphate disodium salt hexahydrate (Sigma).

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3687

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3812

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A8025

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat IgG fraction of antiserum

Sigma-Aldrich

A0418

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

technique(s)

direct ELISA: 1:30,000, western blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:7,000

technique(s)

direct ELISA: 1:20,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40, western blot (chemiluminescent): 1:100,000

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

species reactivity

rabbit

species reactivity

rabbit

species reactivity

rabbit

species reactivity

rabbit

biological source

goat

biological source

goat

biological source

goat

biological source

goat

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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Storage Class

10 - Combustible liquids

wgk

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number
0000548054
0000548054
0000526778
0000526781
0000526778

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Related Content

Data Sheet - A3687

Instructions

Sônia de Fátima Soto et al.
PloS one, 12(8), e0183314-e0183314 (2017-08-19)
Female Wistar rats were exposed to filtered air (F) or to concentrated fine particulate matter (P) for 15 days. After mating, the rats were divided into four groups and again exposed to F or P (FF, FP, PF, PP) beginning
Tanyth E de Gooyer et al.
Investigative ophthalmology & visual science, 47(12), 5553-5560 (2006-11-24)
This study was conducted to evaluate whether regions of the retinal neuropile become hypoxic during periods of high oxygen consumption and whether depletion of the outer retina reduces hypoxia and related changes in gene expression. Retinas from rhodopsin knockout (Rho-/-)
Pingxi Xiao et al.
Experimental and therapeutic medicine, 14(4), 3812-3816 (2017-10-19)
Rheumatic heart disease (RHD) occurs due to the accumulation of complications associated with rheumatic fever, and it results in high morbidity and mortality. The majority of cases of RHD are diagnosed in the chronic stages, when treatment options are limited.
View All Related Papers

Global Trade Item Number

SKUGTIN
A3687-1ML04061837514135
A3687-.25ML04061837514111
A3687-.5ML04061837514128

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