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Anti-Human IgM (μ-chain specific) F(ab′)₂ fragment−Peroxidase antibody produced in goat

Name: Anti-Human IgM (μ-chain specific) F(ab′)<SUB>2</SUB> fragment−Peroxidase antibody produced in goat
Brand: SIGMA
Price: 438 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

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1 mL	Check Cart for Availability	$438.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162464

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

ELISA (d)

Citations:

$438.00

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Properties

biological source

goat

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Goat polyclonal anti-Human IgM (μ-chain specific) F(ab′)2 fragment−Peroxidase antibody is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones Kappa and Bence Jones Lambda myeloma proteins.

Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection.
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3′,5,5′-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu^™ Red into detectable chromophores, light-emitters or fluorescers, respectively.

Immunogen

purified human IgM

Application

Goat polyclonal anti-Human IgM (μ-chain specific) F(ab′)2 fragment−Peroxidase antibody may be used to detect and quantitate the level of IgM in human serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques. ELISA assays on EBV +BCLs established from EBV+ human B cells were performed using HRP conjugated goat anti-human IgM mu chain specific as the secondary. Secondary antibody was incubated for 2 hrs at room temperature.

Peroxidise-conjugated goat F(ab′)2 fragment anti-human IgM (μ-chain specific) has been used as a secondary antibody to detect human IgMs to hantanvirus antigens using ELISA.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Legal Information

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A6907	I0140	SAB4200748
Sigma-Aldrich A4290 Anti-Human IgM (μ-chain specific) F(ab′)₂ fragment−Peroxidase antibody produced in goat	Sigma-Aldrich A6907 Anti-Human IgM (μ-chain specific)−Peroxidase antibody produced in goat	$Anti-Human IgM (μ-chain specific) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich I0140 Anti-Human IgM (μ-chain specific) antibody produced in rabbit	Sigma-Aldrich SAB4200748 Monoclonal Anti-Human IgM (μ-chain specific)-Peroxidase antibody produced in mouse
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description clone MB-11, purified immunoglobulin
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate unconjugated	conjugate peroxidase conjugate
species reactivity human	species reactivity human	species reactivity -	species reactivity human
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form purified immunoglobulin
technique(s) direct ELISA: 1:10,000	technique(s) direct ELISA: 1:10,000	technique(s) indirect ELISA: 1:100,000	technique(s) ELISA: 1:40,000-1:80,000 using 2.5 μg/mL Human IgM for coating
biological source goat	biological source goat	biological source rabbit	biological source mouse
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice

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Pictograms

GHS07,GHS09

Signal Word

Warning

Hazard Codes

H315,H317,H319,H411

Precautionary Statements

P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 3

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