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Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit

Name: Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit
Brand: SIGMA
Price: 254 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Rabbit Anti-Bovine IgG (whole molecule)−HRP

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1 mL	Check Cart for Availability	$254.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162171

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

DB, ELISA (d), IHC (p)

Citations:

$254.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

bovine

technique(s)

direct ELISA: 1:20,000, dot blot: 1:80,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

IgG (immunoglobulin G) contributes to 10-20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.

Immunogen

purified bovine IgG

Application

Anti-Bovine IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in:

enzyme-linked immunosorbent assay (ELISA)
bovine viral diarrhea virus (BVDV) plaque
focus-forming assays

Biochem/physiol Actions

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A8792	A0705	A5545
Sigma-Aldrich A5295 Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit	$Anti-Human IgG (whole molecule)−Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A8792 Anti-Human IgG (whole molecule)−Peroxidase antibody produced in rabbit	Sigma-Aldrich A0705 Anti-Bovine IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit	Sigma-Aldrich A5545 Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description affinity isolated antibody	description affinity isolated antibody, buffered aqueous solution
species reactivity bovine	species reactivity human	species reactivity bovine	species reactivity guinea pig
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate alkaline phosphatase conjugate	conjugate peroxidase conjugate
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form affinity isolated antibody
technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000, dot blot: 1:80,000 (chemiluminescent), direct ELISA: 1:20,000	technique(s) dot blot: 1:80,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150, direct ELISA: 1:40,000	technique(s) dot blot: 1:30,000, direct ELISA: 1:30,000	technique(s) direct ELISA: 1:50,000-1:80,000, dot blot: 1:50,000-1:100,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:800
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C

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Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H317

Precautionary Statements

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

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