This product is a crude enzyme preparation, the activity is not a determined test specification. Historical data indicates activity may range from 10 - 40 units per gram. Please see the link below to review the available assay protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/192/355/a5384enz.pdf
A5384
Aconitase from porcine heart
Synonym(s):
Aconitate hydratase, Citrate (isocitrate) hydrolyase
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 150 mg | Please contact Customer Service for Availability | $627.00 | |
| 250 mg | Please contact Customer Service for Availability | $980.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Biological source:
Porcine heart
$627.00
Please contact Customer Service for Availability
biological source
Porcine heart
Quality Level
form
solid
mol wt
~66 kDa by gel filtration
storage temp.
−20°C
Application
Biochem/physiol Actions
Aconitase catalyses the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle. Aconitase is inhibited by cyanide, sulfide and copper and mercury at low concentrations. It is competitively inhibited by trans-aconitate. Aconitase contains an internal Fe-S cluster which is responsible for its activity.
Analysis Note
Crude preparation containing aconitase activity
Other Notes
One unit will convert 1.0 μmole of citrate (via cis-aconitate) to isocitrate per min at pH 7.4 at 25 °C.
1 of 1
This Item | |||
|---|---|---|---|
| biological source Porcine heart | biological source Porcine heart | biological source Porcine heart | biological source Porcine liver |
| form solid | form solid | form liquid | form ammonium sulfate suspension |
| mol wt ~66 kDa by gel filtration | mol wt - | mol wt - | mol wt 168 kDa |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| A5384-250MG | 04061833423905 |
| A5384-150MG | 04061833616352 |
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