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A6917

Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Rabbit Anti-Horse IgG (whole molecule)−HRP

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1 mL
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$226.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162385
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
18

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biological source

rabbit

Quality Level

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Horse IgG has different subclasses, IgG2a, IgG2b, IgG2c, IgG1 and IgG(T). Compared to other IgG molecules, IgG(T) has more carbohydrate content. In healthy individuals, total IgG is dispersed equally between circulating plasma and interstitial fluids.
Horse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in horse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.

Immunogen

Purified horse IgG

Application

Anti-Horse IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • HeV soluble G
  • indirect ELISA (HeVsG iELISA)

Biochem/physiol Actions

Equine IgG antibodies mainly regulate mucosal and systemic immunological responses and thereby, provide protection against disease-causing pathogens such as Streptococcus equi, and the horse flu virus. Horse IgG may also function to control the advancement of EHV-1 infection .
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 0.05% MIT

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A6917

Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit

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Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit

Anti-Horse IgG (whole molecule)−FITC antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

F7759

Anti-Horse IgG (whole molecule)−FITC antibody produced in rabbit

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

peroxidase conjugate

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

technique(s)

direct ELISA: 1:10,000

technique(s)

direct ELISA: 1:30,000, western blot: 1:30,000

technique(s)

direct ELISA: 1:15,000, dot blot: 1:100,000-1:200,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

technique(s)

direct immunofluorescence: suitable

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous solution

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000533014
0000488042
0000488042
0000533014
0000397792

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Global Trade Item Number

SKUGTIN
A6917-1ML04061837514494
A6917-.25ML04061835805563
A6917-.5ML04061835805570

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