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A7294

Avidin–Alkaline Phosphatase

buffered aqueous solution

Synonym(s):

Avidin–AP

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Pack SizeSKUAvailabilityPrice
1 mL
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$722.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00130582
Form:
buffered aqueous solution
Biological source:
avidin from egg white, enzyme from bovine (calf) intestine

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Quality Level

200

biological source

avidin from egg white, enzyme from bovine (calf) intestine

conjugate

alkaline phosphatase conjugate

form

buffered aqueous solution

technique(s)

direct ELISA: 1:70,000, western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane

shipped in

wet ice

storage temp.

2-8°C

General description

Avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues[1]. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
Avidin is homotetrameric protein (66 kDa) obtained from egg whites and binds strongly to biotin. It has four identical subunits of 16,400 Daltons each. It is an attractive adaptor protein, that is resistant to denaturation. This glycoprotein is present in avian, reptilian and amphibian egg white. The product is affinity purified egg white avidin (acitivity 10-15 units/mg protein) conjugated to alkaline phosphatase using a 0.2% glutaraldehyde method.

Application

Avidin-Alkaline Phosphatase has been used for ELISA[2][3]. The product can also be used for western blot at 1:150,000-1:300,000 dilutions.
Cytokine ELISA Assays were performed using a biotinylated anti-IL-2 antibody and alkaline phosphatase avidin. [4]
Avidin-Alkaline Phosphatase has been used in enzyme-linked immunosorbent assay (ELISA).[2]

Biochem/physiol Actions

Avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities. Avidin is a fatty acid biosynthesis regulator. It participates in terminal cell differentiation by weakening the multiplication of cell without affecting the differentiation process.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Preparation Note

Affinity purified protein conjugated to alkaline phosphatase using 0.2% glutaraldehyde.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
A7419E2636A9275
Avidin–Alkaline Phosphatase buffered aqueous solution

Sigma-Aldrich

A7294

Avidin–Alkaline Phosphatase

Avidin–Peroxidase buffered aqueous solution

Sigma-Aldrich

A7419

Avidin–Peroxidase

ExtrAvidin® –Alkaline Phosphatase buffered aqueous solution

Sigma-Aldrich

E2636

ExtrAvidin® –Alkaline Phosphatase

Avidin from egg white BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

Sigma-Aldrich

A9275

Avidin from egg white

technique(s)

direct ELISA: 1:70,000, western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane

technique(s)

indirect ELISA: 1:100,000, western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well)

technique(s)

dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000

technique(s)

-

biological source

avidin from egg white, enzyme from bovine (calf) intestine

biological source

avidin from egg white, enzyme from horseradish

biological source

-

biological source

egg white

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

dry ice

shipped in

ambient

shipped in

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000561199
0000519186
0000519186
0000332011
0000332011

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Global Trade Item Number

SKUGTIN
A7294-1ML04061838160867

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