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A7294

Avidin–Alkaline Phosphatase

buffered aqueous solution

Synonym(s):

Avidin–AP

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Size/SKUAvailabilityPrice
1 mL

Available to ship TODAYfromMILWAUKEE

$518.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00130582
Form:
buffered aqueous solution
Biological source:
avidin from egg white, enzyme from bovine (calf) intestine

$518.00

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Quality Segment

biological source

avidin from egg white, enzyme from bovine (calf) intestine

conjugate

alkaline phosphatase conjugate

form

buffered aqueous solution

technique(s)

direct ELISA: 1:70,000, western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane

shipped in

wet ice

storage temp.

2-8°C

General description

Avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
Avidin is homotetrameric protein (66 kDa) obtained from egg whites and binds strongly to biotin. It has four identical subunits of 16,400 Daltons each. It is an attractive adaptor protein, that is resistant to denaturation. This glycoprotein is present in avian, reptilian and amphibian egg white. The product is affinity purified egg white avidin (acitivity 10-15 units/mg protein) conjugated to alkaline phosphatase using a 0.2% glutaraldehyde method.

Application

Avidin-Alkaline Phosphatase has been used for ELISA. The product can also be used for western blot at 1:150,000-1:300,000 dilutions.
Avidin-Alkaline Phosphatase has been used in enzyme-linked immunosorbent assay (ELISA).
Cytokine ELISA Assays were performed using a biotinylated anti-IL-2 antibody and alkaline phosphatase avidin.

Biochem/physiol Actions

Avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities. Avidin is a fatty acid biosynthesis regulator. It participates in terminal cell differentiation by weakening the multiplication of cell without affecting the differentiation process.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Preparation Note

Affinity purified protein conjugated to alkaline phosphatase using 0.2% glutaraldehyde.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Avidin–Alkaline Phosphatase buffered aqueous solution

Sigma-Aldrich

A7294

Avidin–Alkaline Phosphatase

Avidin–Peroxidase buffered aqueous solution

Sigma-Aldrich

A7419

Avidin–Peroxidase

Avidin–Peroxidase lyophilized powder

Sigma-Aldrich

A3151

Avidin–Peroxidase

ExtrAvidin® –Alkaline Phosphatase buffered aqueous solution

Sigma-Aldrich

E2636

ExtrAvidin® –Alkaline Phosphatase

description

buffered aqueous solution

description

buffered aqueous solution

description

lyophilized powder

description

buffered aqueous solution

technique(s)

western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane, direct ELISA: 1:70,000

technique(s)

western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well), indirect ELISA: 1:100,000

technique(s)

direct ELISA: 1:50,000

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, dot blot: 1:300,000, indirect ELISA: 1:60,000

biological source

enzyme from bovine (calf) intestine, avidin from egg white

biological source

enzyme from horseradish, avidin from egg white

biological source

enzyme from horseradish, avidin from egg white

biological source

-

form

buffered aqueous solution

form

buffered aqueous solution

form

lyophilized powder

form

buffered aqueous solution

shipped in

wet ice

shipped in

dry ice

shipped in

-

shipped in

ambient

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

conjugate

alkaline phosphatase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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