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A7419

Avidin–Peroxidase

buffered aqueous solution

Synonym(s):

Avidin–HRP

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Size/SKUAvailabilityPrice
2 mL

Available to ship TODAYfromMILWAUKEE

$379.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00130587
Form:
buffered aqueous solution
Biological source:
avidin from egg white, enzyme from horseradish

$379.00

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Quality Segment

biological source

avidin from egg white, enzyme from horseradish

conjugate

peroxidase conjugate

form

buffered aqueous solution

technique(s)

indirect ELISA: 1:100,000, western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well)

shipped in

dry ice

storage temp.

−20°C

General description

Avidin is homotetrameric protein (68kDa) obtained from egg whites and binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin-Peroxidase has been used for ELISA applications. The product can also be used for western blot applications at dilutions ranging from 1:400,000 to 1:800,000.
Western blot analysis of proteins in HUVEC cells that had underwent cell surface biotinylation were detected using HRP-conjugated avidin at 33.3 ng/ml.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Preparation Note

Affinity purified protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
A9275A3151A4541
Avidin–Peroxidase buffered aqueous solution

Sigma-Aldrich

A7419

Avidin–Peroxidase

Avidin from egg white BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

Sigma-Aldrich

A9275

Avidin from egg white

Avidin–Peroxidase lyophilized powder

Sigma-Aldrich

A3151

Avidin–Peroxidase

Anti-Biotin−Peroxidase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A4541

Anti-Biotin−Peroxidase antibody produced in goat

description

buffered aqueous solution

description

BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

description

lyophilized powder

description

affinity isolated antibody, buffered aqueous solution

technique(s)

western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well), indirect ELISA: 1:100,000

technique(s)

-

technique(s)

direct ELISA: 1:50,000

technique(s)

direct ELISA: 1:2,000

biological source

enzyme from horseradish, avidin from egg white

biological source

egg white

biological source

avidin from egg white, enzyme from horseradish

biological source

goat

form

buffered aqueous solution

form

lyophilized powder

form

lyophilized powder

form

buffered aqueous solution

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

dry ice

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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