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A7650

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Rabbit Anti-Goat IgG (whole molecule)–AP

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1 mL

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$380.00
$323.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162335
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
16

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biological source

rabbit

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:7,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

An immunoglobulin has two heavy chains and two light chains connected by disulfide bond.(5) It is a glycoprotein.IgG is a major class of immunoglobulin.(6) Goat IgG has two subclasses- IgG1 and IgG2.(7)
Second antibodies (secondary antibodies) are antibodies raised against other antibodies, typically from another species, called primary antibodies. Primary antibodies are generated with and bind to specific antigens to create antigen antibody complexes which may be detected in a variety of immunochemical and immunohistochemical configurations involving soluble or insoluble signaling molecules. The immunodetection system depends upon the binding of a signal competent secondary antibody to a primary antibody:antigen complex.
The suitability of any secondary antibody to a specific immunodetection application is dependent upon its signal development system configuration. Signal development systems are based upon the chemical conjugation of a signal mediator to the secondary antibody. Major secondary antibody signal systems are color (chromogenic), chemiluminescence or fluorescence based and involve the use of enzymes or high affinity binary systems such as biotin:avidin.

Immunogen

Antiserum is produced in rabbit using purified goat IgG as the immunogen

Application

Anti-Goat IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit has been used in western blotting.[1]
Anti-Goat IgG (whole molecule)-Alkaline Phosphatase antibody produced in rabbit has been used in immunoblotting and enzyme-linked immunosorbent assay (ELISA).
Immunocytochemistry was performed on cells fixed in 4% paraformaldhyde for 15 minutes using alkaline phosphatase-conjugated rabbit anti-goat IgG diluted 1:1000 in TBS as the secondary antibody.

Biochem/physiol Actions

Antiserum is determined to be immunospecific for goat IgG by immunoelectrophoresis (IEP), prior to conjugation, versus goat IgG and normal goat serum.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to the fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulin G (IgG) mainly helps in immune defense.(5) IgG participates in hypersensitivity type II and type III.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine, 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.

Preparation Note

Store at 2-8°C.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A7650

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A4062

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A6066

Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A4187

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

technique(s)

direct ELISA: 1:7,000

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100, western blot: 1:30,000-1:60,000

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000487221
0000487221
0000460734
0000460734
0000405515

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Related Content

Data Sheet - A7650

Instructions

MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing loss
Zhuang W, et al.
International Journal of Molecular Medicine, 41(6), 3448-3456 (2018)
Yalan Li et al.
Antioxidants & redox signaling, 38(1-3), 115-136 (2022-06-17)
Aims: Noise damage to auditory hair cells is associated with oxidative stress and mitochondrial dysfunction. This study aimed to investigate the possible effect of sestrin 2 (SESN2), an endogenous antioxidant protein, on noise-induced hearing loss (NIHL) and the underlying mechanisms.
The significance of complement in proliferative vitreoretinopathy.
Grisanti S, et al.
Investigative Ophthalmology & Visual Science, 32(10), 2711-2717 (1991)
View All Related Papers

Global Trade Item Number

SKUGTIN
A7650-.5ML04061835805679
A7650-.25ML04061835805662
A7650-1ML04061833386002
A7650-5X1ML04061835805686

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