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A8792

Anti-Human IgG (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

HRP Rabbit Anti-Human IgG (whole molecule)

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2 mL

Available to ship TODAYfromMILWAUKEE

$337.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162456
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p)
Citations:
29

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biological source

rabbit

Quality Segment

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

direct ELISA: 1:40,000, dot blot: 1:80,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 percent of the total immunoglobulin consists of IgG.

Immunogen

purified human IgG

Application

Anti-Human IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in:
enzyme-linked immunosorbent assay (ELISA)
western blotting
microarray analysis
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.
Rabbit Anti-Human IgG (whole molecule)-Peroxidase antibody binds to human IgG.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Human IgG (whole molecule)−Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

A8792

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Sigma-Aldrich

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Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

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Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

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Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit

description

IgG fraction of antiserum, buffered aqueous solution

description

IgG fraction of antiserum, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous solution

description

IgG fraction of antiserum, buffered aqueous solution

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

species reactivity

human

species reactivity

human

species reactivity

guinea pig

species reactivity

-

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

biological source

rabbit

biological source

goat

biological source

rabbit

biological source

rabbit

technique(s)

dot blot: 1:80,000 (chemiluminescent), direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

technique(s)

dot blot: 1:80,000, direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

technique(s)

dot blot: 1:50,000-1:100,000 (chemiluminescent), direct ELISA: 1:50,000-1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:800

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150, direct ELISA: 1:15,000, dot blot: 1:100,000-1:200,000 (chemiluminescent)

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

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Questions

  1. what is the recommended dilution for western blotting

    1 answer

    1. The Western Blot picture is from the article in link below. However, It does not mention how the product is diluted:
      https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0026032&type=printable

      Below are links to articles that have cited this antibody in Western Blot. Various dilutions are used. The end-user may titrate the product to find the best dilution for their application. The end-user may try something like 1:1000, 1:5000, 1:10,000, 1:20,000, etc.:

      https://www.researchgate.net/profile/Yuriy-Kit/publication/303736655_Identification_of_the_Unique_Properties_of_IgGs_and_their_Heavy_chains_in_Blood_serum_of_Multiple_Sclerosis_Patients/links/574ff60608aef199238f01e4/Identification-of-the-Unique-Properties-of-IgGs-and-their-Heavy-chains-in-Blood-Serum-of-Multiple-Sclerosis-Patients.pdf

      https://www.scielo.br/j/jaos/a/pBt9nVwJydK6Qz3qwMrNLrc/?lang=en

      https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.612781/full

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