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A9037

Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti Rat, Anti Rat Hrp

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1 mL
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$222.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162813
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
96

$222.00

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biological source

goat

Quality Level

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG conjugated to a detectable substrate is a useful tool for the analysis of target proteins. Anti-Rat IgG (whole molecule)-Peroxidase antibody binds all rat immunoglobulins.
IgG (immunoglobulin γ) is a monomeric antibody containing a γ Fc region as a part of heavy chain, which is linked to the Fab arm through a hinge region. It is the most abundant serum antibody and constitutes to 70% of the total immunoglobulins.

Immunogen

Purified rat IgG

Application

Anti-Rat IgG (whole molecule)-Peroxidase antibody is suitable for use in ELISA and immunoblot at 1:5000 dilutions.
Lysates generated from primary cells isolated from lymphoid organs of P. monodon shrimp were analyzed by western blot using HRP-conjugated goat anti-rat as the secondary antibody at a 1:4000 dilution.

Biochem/physiol Actions

IgG (immunoglobulin γ), upon epitope binding, mediates the activation of classical complement pathway. Interaction between the host cell membrane and IgG antibody results in type II hypersensitivity reaction. Decreased serum levels of IgG contributes to common variable immunodeficiency, thus making the individual easily prone to infections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
A5795A8438A6066
Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A9037

Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in goat

Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A5795

Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit

Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A8438

Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A6066

Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

biological source

goat

biological source

rabbit

biological source

goat

biological source

rabbit

technique(s)

direct ELISA: 1:10,000

technique(s)

direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:750, western blot (chemiluminescent): 1:80,000-1:160,000 using rat PC12 cell extract

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (frozen sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100, western blot: 1:30,000-1:60,000

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

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pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000538946
0000526801
0000526801
0000538946
0000512088

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Global Trade Item Number

SKUGTIN
A9037-.25ML04061835805808
A9037-.5ML04061835805815
A9037-1ML04061833401217

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