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Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in goat

Name: Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in goat
Brand: SIGMA
Price: 169 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti Rat, Anti Rat Hrp

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Size/SKU	Availability	Price
1 mL	Check Cart for Availability	$169.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162813

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

ELISA (d)

Citations:

$169.00

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Properties

biological source

goat

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG conjugated to a detectable substrate is a useful tool for the analysis of target proteins. Anti-Rat IgG (whole molecule)-Peroxidase antibody binds all rat immunoglobulins.
IgG (immunoglobulin γ) is a monomeric antibody containing a γ Fc region as a part of heavy chain, which is linked to the Fab arm through a hinge region. It is the most abundant serum antibody and constitutes to 70% of the total immunoglobulins.

Immunogen

Purified rat IgG

Application

Anti-Rat IgG (whole molecule)-Peroxidase antibody is suitable for use in ELISA and immunoblot at 1:5000 dilutions.

Lysates generated from primary cells isolated from lymphoid organs of P. monodon shrimp were analyzed by western blot using HRP-conjugated goat anti-rat as the secondary antibody at a 1:4000 dilution.

Biochem/physiol Actions

IgG (immunoglobulin γ), upon epitope binding, mediates the activation of classical complement pathway. Interaction between the host cell membrane and IgG antibody results in type II hypersensitivity reaction. Decreased serum levels of IgG contributes to common variable immunodeficiency, thus making the individual easily prone to infections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A5545	A6154	A8275
Sigma-Aldrich A9037 Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in goat	Sigma-Aldrich A5545 Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit	Sigma-Aldrich A6154 Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat	$Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A8275 Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum
technique(s) direct ELISA: 1:10,000	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:800, direct ELISA: 1:50,000-1:80,000, dot blot: 1:50,000-1:100,000 (chemiluminescent)	technique(s) direct ELISA: 1:10,000	technique(s) direct ELISA: 1:10,000
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate
biological source goat	biological source rabbit	biological source goat	biological source goat
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution

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Pictograms

GHS07,GHS09

Signal Word

Warning

Hazard Codes

H315,H317,H319,H411

Precautionary Statements

P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 3

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