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A9042

Anti-Dog IgG (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Rabbit Anti-Dog IgG (whole molecule)−HRP

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2 mL
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$337.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162268
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d)
Citations:
6

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biological source

rabbit

Quality Level

200

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:25,000, dot blot: 1:40,000 using direct chemiluminescence assay using 10 ng dog IgG/dot. Luminol plus enhancer was used as substrate.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Immunoglobulins (Igs) belong to immunoglobulin super-family. IgG is abundant immunoglobulin in proteins in human serum. The four classes of IgG can be IgG1, IgG2, IgG3 and IgG4.

Immunogen

Dog IgG

Application

Anti-Dog IgG (whole molecule)-peroxidase antibody has been used in
  • enzyme linked immunosorbent assay (ELISA)[1]
  • IgG/dot
  • western blotting

Biochem/physiol Actions

Anti-dog IgG antibody is specific for IgG in dogs.
Dog IgG is a glycoprotein antibody that mediates intestinal antibacterial defense mechanisms. It may regulate the phagocytosis of Gram-negative bacteria . Anti-dog IgG antibody is specific for IgG in dogs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Sigma-Aldrich

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Anti-Dog IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

technique(s)

direct ELISA: 1:25,000, dot blot: 1:40,000 using direct chemiluminescence assay using 10 ng dog IgG/dot. Luminol plus enhancer was used as substrate.

technique(s)

direct ELISA: 1:10,000

technique(s)

direct ELISA: 1:40,000, dot blot: 1:150,000 (chemiluminescent)

technique(s)

direct ELISA: 1:6,000, dot blot: 1:4,000, western blot (chemiluminescent): 1:4,000

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000552003
0000543850
0000543850
0000552003
0000340616

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R J Heddle et al.
Immunology, 29(1), 197-208 (1975-07-01)
Secretory IgA antibodies to Vibrio cholerae were purified from the parotid saliva and mammary secretions of locally and orally immunized dogs using gel filtration, ion-exchange chromatography and anti-immunoglobulin immuno-absorbents. IgM and IgG antibodies were isolated from serum by gel filtration
Xiaohui Yao et al.
Frontiers in cellular and infection microbiology, 13, 1291937-1291937 (2024-01-18)
The Hedi virus (HEDV) and Wuxiang virus (WUXV) are newly discovered Bunyaviruses transmitted by sandflies. The geographical distribution of isolation of these two viruses continues to expand and it has been reported that WUXV causes neurological symptoms and even death
Characterization of the Major Antigenic Protein 2 of Ehrlichia canis and Ehrlichia chaffeensis and Its Application for Serodiagnosis of Ehrlichiosis.
Knowles, T., et al.
Clinical and Vaccine Immunology : CVI, 10(4), 520-524 (2003)
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Global Trade Item Number

SKUGTIN
A9042-2ML04061833401231

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