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Anti-Goat/Sheep IgG−Peroxidase antibody, Mouse monoclonal

Name: Anti-Goat/Sheep IgG−Peroxidase antibody, Mouse monoclonal
Brand: SIGMA
Price: 323 USD
Availability: InStock

clone GT-34, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Goat/Sheep IgG

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Size/SKU	Availability	Price
1 vial	Estimated to ship onNovember 11, 2026fromMILWAUKEE	$323.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

peroxidase conjugate

Clone:

GT-34, monoclonal

Application:

DB, ELISA (d), IHC (p)

Citations:

$323.00

Estimated to ship onNovember 11, 2026Details

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Properties

biological source

mouse

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

GT-34, monoclonal

form

lyophilized powder

species reactivity

sheep, goat, bovine

should not react with

rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human

packaging

vial of 0.5 mL

technique(s)

direct ELISA: 1:30,000, dot blot: 1:160,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

isotype

IgG1

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

IgG is the most abundant antibody in human serum.It is found in blood and secreted by the B cells. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are connected by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.

IgGs are glycoprotein antibodies that modulate several immune responses. Goat or sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-goat/sheep IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody shows strong cross-reactivity with sheep IgG and cross-reacts with bovine IgG. No cross-reaction of the unconjugated monoclonal antibody is observed with human serum and tissue components. Moreover, the antibody does not react with the following species: guinea pig, rat, mouse, rabbit, horse, dog, chicken, pig and cat.

The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.

Application

ELISA using HRP-conjugated monoclonal anti-goat/sheep IgG as the secondary was performed on human T cells.

Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody has been used for western blot (1:5,000) and ELISA. The product can also be used for dot blot (1:30,000) and IHC (1:100).

Biochem/physiol Actions

IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC). IgG enables transplacental transport. It has the longest serum half life. It might be associated with allergy.

Physical form

Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A1949	A6782	SAB3701095
Sigma-Aldrich A9452 Anti-Goat/Sheep IgG−Peroxidase antibody, Mouse monoclonal	Sigma-Aldrich A1949 Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal	Sigma-Aldrich A6782 Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in sheep	Sigma-Aldrich SAB3701095 Anti-Mouse IgG (H+L)-Peroxidase antibody produced in sheep
description clone GT-34, purified from hybridoma cell culture	description clone RG-96, purified from hybridoma cell culture	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, lyophilized powder
species reactivity sheep, goat, bovine	species reactivity rabbit	species reactivity -	species reactivity mouse
antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form affinity isolated antibody
technique(s) dot blot: 1:160,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, direct ELISA: 1:30,000	technique(s) direct ELISA: 1:40,000, western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200	technique(s) direct ELISA: 1:10,000	technique(s) indirect ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
biological source mouse	biological source mouse	biological source sheep	biological source sheep
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate
isotype IgG1	isotype IgG1	isotype -	isotype -

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Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H317

Precautionary Statements

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

13 - Non Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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