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Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

Name: Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat
Brand: SIGMA
Price: 210 CAD

affinity isolated antibody, buffered aqueous solution

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Pack Size	Availability	Price
0.25 mL	Please contact Customer Service for Availability	$210.00
0.5 mL	Please contact Customer Service for Availability	$318.00
1 mL	Please contact Customer Service for Availability	$580.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162782

Conjugate:

alkaline phosphatase conjugate

Clone:

polyclonal

Application:

ELISA (d), IHC (p), WB

Citations:

$210.00

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Properties

biological source

goat

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rabbit

technique(s)

direct ELISA: 1:45,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot: 1:100,000-1:200,000 using detecting actin in total cell extract of HeLa cells (5-10 μg per well)

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Binds all rabbit Igs.

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.

Immunoglobulin G (IgG) belongs to the immunoglobulin family. It is widely expressed and consists of a gamma (γ) heavy chain in the constant (C) region. IgG is a monomer with a molecular weight of about 150 kDa. The structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa respectively. The primary structure of the antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. The Fc fragment contains the C_H2 and C_H3 domains of the antibody molecule.

Immunogen

purified rabbit IgG

Application

Citrullination of antithrombin by PADI4 was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary diluted at 1:5000 in 0.05M carbonate/bicarbonate buffer (Ph 9.6).

Goat Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody has been used for immunohistochemistry and western blot applications. The antibody can also be used for direct ELISA at 1:45,000.

Lysates of human fibroblasts and Hela cells were analyzed by western blot using alkaline phosphatase-conjugated goat anti-rabbit IgG as the secondary antibody at a 1:30000 dilution.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide as preservative

Other Notes

Antibody adsorbed with human IgG.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A3812	A0418	A8025
Sigma-Aldrich A9919 Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A3812 Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat	$Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat IgG fraction of antiserum$ Sigma-Aldrich A0418 Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A8025 Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat
conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody
biological source goat	biological source goat	biological source goat	biological source goat
species reactivity rabbit	species reactivity rabbit	species reactivity rabbit	species reactivity rabbit
technique(s) direct ELISA: 1:45,000, western blot: 1:100,000-1:200,000 using detecting actin in total cell extract of HeLa cells (5-10 μg per well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100	technique(s) direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000	technique(s) direct ELISA: 1:20,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40, western blot (chemiluminescent): 1:100,000	technique(s) direct ELISA: 1:7,000
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
A9919-.5ML	04061837514777
A9919-1ML	04061833413234
A9919-.25ML	04061833413210

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