This product has a potency specification of 1.5-2.0 units per mg. The exact value will be listed on the lot specific Certificate of Analysis. See the link below to review a sample Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/B2434-BULKSLCM6973.pdf
B2434
Bleomycin sulfate from Streptomyces verticillus
BioXtra, crystalline
Bleomycin sulfate from Streptomyces verticillus
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Synonym(s):
Blenoxane, Bleo, Blexane
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 20 mg | Available to ship TODAYfromMILWAUKEE | $1,620.00 |
About This Item
CAS Number:
NACRES:
NA.85
PubChem Substance ID:
UNSPSC Code:
51111733
EC Number:
232-925-2
MDL number:
product line
BioXtra
Quality Level
form
crystalline
potency
1.5-2.0 units per mg
technique(s)
cell culture | mammalian: suitable
solubility
H2O: 20 mg/mL, clear, colorless
cation traces
Cu: ≤0.10%
antibiotic activity spectrum
Gram-negative bacteria, Gram-positive bacteria, neoplastics
mode of action
DNA synthesis | interferes
storage temp.
2-8°C
SMILES string
[O-]S([O-])(=O)=O.C[C@@H](O)[C@@H](NC(=O)[C@@H](C)[C@H](O)[C@@H](C)NC(=O)[C@H](NC(=O)c1nc(nc(N)c1C)[C@H](CC(N)=O)NC[C@H](N)C(N)=O)[C@@H](O[C@@H]2O[C@@H](CO)[C@@H](O)[C@H](O)[C@@H]2O[C@@H]3O[C@H](CO)[C@@H](O)[C@@H](OC(N)=O)[C@@H]3O)c4c[nH]cn4)C(=O)NCCc5nc(cs5)-c6ncc(s6)C(=O)NCCC[S+](C)C.C[C@@H](O)[C@@H](NC(=O)[C@@H](C)[C@H](O)[C@@H](C)NC(=O)[C@H](NC(=O)c7nc(nc(N)c7C)[C@H](CC(N)=O)NC[C@H](N)C(N)=O)[C@@H](O[C@@H]8O[C@@H](CO)[C@@H](O)[C@H](O)[C@@H]8O[C@@H]9O[C@H](CO)[C@@H](O)[C@@H](OC(N)=O)[C@@H]9O)c%10c[nH]cn%10)C(=O)NCCc%11nc(cs%11)-c%12ncc(s%12)C(=O)NCCC[S+](C)C
InChI
1S/2C55H83N17O21S3.H2O4S/c2*1-20-33(69-46(72-44(20)58)25(12-31(57)76)64-13-24(56)45(59)82)50(86)71-35(41(26-14-61-19-66-26)91-54-43(39(80)37(78)28(16-73)90-54)92-53-40(81)42(93-55(60)88)38(79)29(17-74)89-53)51(87)67-22(3)36(77)21(2)47(83)70-34(23(4)75)49(85)63-10-8-32-68-27(18-94-32)52-65-15-30(95-52)48(84)62-9-7-11-96(5)6;1-5(2,3)4/h2*14-15,18-19,21-25,28-29,34-43,53-54,64,73-75,77-81H,7-13,16-17,56H2,1-6H3,(H13-,57,58,59,60,61,62,63,66,67,69,70,71,72,76,82,83,84,85,86,87,88);(H2,1,2,3,4)/t2*21-,22+,23+,24-,25-,28-,29+,34+,35+,36-,37+,38+,39-,40-,41-,42+,43-,53-,54-;/m00./s1
InChI key
OOXTWFJZZAJGKA-CNLAFNBISA-N
General description
Chemical structure: glycopeptide
Application
Bleomycin Sulfate is an antineoplastic antibiotic isolated from Streptomyces verticillus. It is recommended for use as a cell culture agent at 10-100 μg/mL.
Biochem/physiol Actions
Bleomycin sulfate binds to DNA, causes ssDNA scission at specific base sequences and inhibits DNA synthesis. This inhibitory action requires bleomycin to bind oxygen and a metal ion. It can also cleave RNA, to a lesser degree but more selectively. It acts as an inducer and regulator of apoptosis and inhibits tumor angiogenesis.
Preparation Note
The product is soluble in water in water at 20 mg/mL and is active for several days. A solution of 2 units/mL in 0.1 M neutral potassium phosphate should be used within 14 days. A solution is active for three months at 3 units/mL in normal saline.
Other Notes
Keep container tightly closed in a dry and well-ventilated place, strongly hygroscopic
This product is a mixture of glycopeptides antibiotics containing Bleomycin A2 (70%) and B2(30%). Bleomycins differ from each other in the terminal amine and show varying biological activity.
Disclaimer
This product should be stored at 2-8°C.
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) - |
| antibiotic activity spectrum Gram-negative bacteria, neoplastics, Gram-positive bacteria | antibiotic activity spectrum fungi | antibiotic activity spectrum - | antibiotic activity spectrum - |
| product line BioXtra | product line - | product line - | product line - |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level - |
| form crystalline | form crystalline | form lyophilized powder ( | form - |
| potency 1.5-2.0 units per mg | potency - | potency - | potency - |
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signalword
Danger
hcodes
Hazard Classifications
Carc. 2 - Muta. 1B - Repr. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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Articles
DNA damage and repair mechanism is vital for maintaining DNA integrity. Damage to cellular DNA is involved in mutagenesis, the development of cancer among others.
Z G Fridlender et al.
The European respiratory journal, 30(2), 205-213 (2007-05-17)
Epithelial cell injury and apoptosis are recognised as early features in idiopathic pulmonary fibrosis and bleomycin-induced fibrosis in mice. Telomerase is a known apoptosis-alleviating factor. The role of telomerase was studied during bleomycin-induced lung epithelial cell (LEC) apoptosis in vitro
Abdulaleem Alnajar et al.
PloS one, 8(11), e81356-e81356 (2013-11-22)
Fibrogenesis is usually initiated when regenerative processes have failed and/or chronic inflammation occurs. It is characterised by the activation of tissue fibroblasts and dysregulated synthesis of extracellular matrix proteins. FHL2 (four-and-a-half LIM domain protein 2) is a scaffolding protein that
Andrea M Foskett et al.
American journal of physiology. Lung cellular and molecular physiology, 306(2), L120-L131 (2013-11-19)
Previous reports demonstrated that bleomycin-induced injury of lungs in mice can be improved by the administration of murine multipotent adult stem/progenitor cells (MSCs) from the bone marrow. Recently some of the beneficial effects of MSCs have been explained by the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B2434-20MG | 04061833426791 |
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I can't find an expiration or use by date for the powder. Is it better to resuspend and freeze upon arrival or is it better to keep at 4deg until ready to resuspend and freeze?
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This product does not contain an expiration date or recommended retest date in its crystalline format. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment.
This product should be placed in the proper storage temperature of 2-8°C upon arrival. Once reconstituted, the material should be stored at 2-8°C as well. For reconstitution instructions and stability, please see "Preparation Notes" section of the product page.
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