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B5645

Anti-Bovine IgG (whole molecule) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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1 mL
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314,00 $

A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162167
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA (i)
Citations:
14

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biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

indirect ELISA: 1:20,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgG (immunoglobulin G) contributes to 10-20% of plasma protein and is regarded as one of the most predominant serum proteins. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.
Immunoglobulin G (IgG) is the most important type of glycoprotein antibody present in human serum. It is found in blood and has four polypeptide chains.The light and heavy chains are interconnected by disulfide bonds. IgG has the longest serum half-life when compared to other immunoglobulins.

Application

Anti-Bovine IgG (whole molecule) antibody produced in rabbit has been used to coat immulon 4HBX plates in serum antibody enzyme-linked immunosorbent assay (ELISA) and western blotting.
Anti-Bovine IgG (whole molecule) antibody produced in rabbit is suitable for ELISA at a working dilution of 1:20,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000.

Biochem/physiol Actions

Immunoglobulin G (IgG) initiates the classical pathway of the complement system and also nullifies virus particles and toxins. Maternal IgG is transferred to the fetus through the placenta that is critical for the immune defense of the neonate against infections. IgG modulates antibody-dependent cell-mediated cytotoxicity (ADCC) and might be associated with allergy. Digestion of IgG by papain releases fragment antigen-binding (Fab) and pepsin digestion of IgG produces crystallizable (fc) fragment and a single dimeric F(ab)2. IgG levels in severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infected patients may be used to evaluate the severity and prognosis of coronavirus disease 2019 (COVID-19).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Goat IgG (whole molecule) antibody produced in rabbit

description

affinity isolated antibody, buffered aqueous solution

description

IgG fraction of antiserum, buffered aqueous solution

description

whole antiserum

description

affinity isolated antibody, buffered aqueous solution

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

whole antiserum

antibody form

affinity isolated antibody

technique(s)

indirect ELISA: 1:20,000

technique(s)

indirect ELISA: 1:150,000, quantitative precipitin assay: 2.0 mg/mL

technique(s)

indirect ELISA: 1:150,000, quantitative precipitin assay: 4.0 mg/mL

technique(s)

indirect ELISA: 1:60,000, quantitative precipitin assay: 2.0 mg/mL

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

buffered aqueous solution

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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