B5645
Anti-Bovine IgG (whole molecule) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 mL | Available to ship TODAYfromMILWAUKEE | $238.00 |
About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA (i)
Citations:
14
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
indirect ELISA: 1:20,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
IgG (immunoglobulin G) contributes to 10-20% of plasma protein and is regarded as one of the most predominant serum proteins. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.
Immunoglobulin G (IgG) is the most important type of glycoprotein antibody present in human serum. It is found in blood and has four polypeptide chains.The light and heavy chains are interconnected by disulfide bonds. IgG has the longest serum half-life when compared to other immunoglobulins.
Application
Anti-Bovine IgG (whole molecule) antibody produced in rabbit has been used to coat immulon 4HBX plates in serum antibody enzyme-linked immunosorbent assay (ELISA) and western blotting.
Anti-Bovine IgG (whole molecule) antibody produced in rabbit is suitable for ELISA at a working dilution of 1:20,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000.
Biochem/physiol Actions
Immunoglobulin G (IgG) initiates the classical pathway of the complement system and also nullifies virus particles and toxins. Maternal IgG is transferred to the fetus through the placenta that is critical for the immune defense of the neonate against infections. IgG modulates antibody-dependent cell-mediated cytotoxicity (ADCC) and might be associated with allergy. Digestion of IgG by papain releases fragment antigen-binding (Fab) and pepsin digestion of IgG produces crystallizable (fc) fragment and a single dimeric F(ab)2. IgG levels in severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infected patients may be used to evaluate the severity and prognosis of coronavirus disease 2019 (COVID-19).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| antibody form affinity isolated antibody | antibody form IgG fraction of antiserum | antibody form whole antiserum | antibody form affinity isolated antibody |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source rabbit |
| technique(s) indirect ELISA: 1:20,000 | technique(s) indirect ELISA: 1:150,000, quantitative precipitin assay: 2.0 mg/mL | technique(s) indirect ELISA: 1:150,000, quantitative precipitin assay: 4.0 mg/mL | technique(s) indirect ELISA: 1:60,000, quantitative precipitin assay: 2.0 mg/mL |
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
Katrin Spiesberger et al.
PloS one, 10(10), e0140471-e0140471 (2015-10-16)
30 years ago, the potential of bispecific antibodies to engage cytotoxic T cells for the lysis of cancer cells was discovered. Today a variety of bispecific antibodies against diverse cell surface structures have been developed, the majority of them produced
Low expression of secreted frizzled-related protein 2 and nuclear accumulation of beta-catenin in aggressive nonfunctioning pituitary adenoma
Wu Y, et al.
Oncology Letters, 12(1), 199-206 (2016)
Formalin-inactivated bovine RSV vaccine enhances a Th2 mediated immune response in infected cattle
Kalina WV, et al.
Vaccine, 22(11-12), 1465-1474 (2004)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B5645-1ML | 04061837514920 |