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C0150

Cathepsin B from human placenta

lyophilized powder, ≥5 units/mg protein

Synonym(s):

Cathepsin B1

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Size/SKUAvailabilityPrice
2 units

Estimated to ship onSeptember 10, 2026fromMILWAUKEE

$95.80
10 units

Estimated to ship onSeptember 03, 2027fromMILWAUKEE

$382.00

About This Item

CAS Number:
9047-22-7
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.4.22.1 (BRENDA, IUBMB)
MDL number:
MFCD00130748
Specific activity:
≥5 units/mg protein

$95.80

Estimated to ship onSeptember 10, 2026Details

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form

lyophilized powder

Quality Segment

200

specific activity

≥5 units/mg protein

mol wt

24.5 kDa

composition

Protein, ~50% Lowry

solubility

H2O: soluble 1.0 mg/mL, clear

UniProt accession no.

P07858

storage temp.

−20°C

Gene Information

human ... CTSB(1508)

General description

Cathepsin B is encoded by the gene CTSB and is located in the human chromosome at 8p23.1. Cathepsin B is a lysosomal proteolytic enzyme of cysteine protease family. It is present in all mammalian cells. It is essential for the intracellular protein turnover.

Application

Cathepsin B is a lysosomal cysteine proteinase which hydrolyzes proteins with a broad specificity for peptide bonds. Cathepsin B may be a useful tool in Alzheimer′s research, as it may have a role in the natural defense against the disease . Cathepsin B may be used to cleave procaspase 1 and procaspase 11, and to induce apoptosis in digitonin-permeabilized cells.
Cathepsin B from human placenta has been used in the in vitro digestion of modified myelin oligodendrocyte glycoprotein (MOG). It has also been used in determining the effect of cysteine protease inhibitors.

Biochem/physiol Actions

Cathepsin B preferentially cleaves at the caboxyl side of Arg-Arg bonds in small molecule substrates. Cathepsin B has been shown to degrade β-amyloid precursor protein into harmless fragments . Cathepsin B inhibition has been shown to limit bone metastasis in breast cancer .
Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Physical form

Lyophilized powder containing phosphate buffer salts

Preparation Note

Dissolves in water at 1 mg/mL concentration, yieliding a clear solution.

Other Notes

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

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This Item
C4428SRP0293
Cathepsin B from human placenta lyophilized powder, ≥5 units/mg protein

Sigma-Aldrich

C0150

Cathepsin B from human placenta

Cathepsin G from human leukocytes lyophilized powder, ≥60 units/mg protein (Bradford)

Sigma-Aldrich

C4428

Cathepsin G from human leukocytes

Cathepsin V Active human recombinant, expressed in FreeStyle™ 293-F cells, ≥90% (SDS-PAGE)

Sigma-Aldrich

SRP0293

Cathepsin V Active human

description

lyophilized powder, ≥5 units/mg protein

description

lyophilized powder, ≥60 units/mg protein (Bradford)

description

recombinant, expressed in FreeStyle™ 293-F cells, ≥90% (SDS-PAGE)

specific activity

≥5 units/mg protein

specific activity

≥60 units/mg protein (Bradford)

specific activity

≥436 pmol/min-μg

Gene Information

human ... CTSB(1508)

Gene Information

human ... CTSG(1511)

Gene Information

human ... CTSL2(1515)

form

lyophilized powder

form

lyophilized powder

form

aqueous solution

solubility

H2O: soluble 1.0 mg/mL, clear

solubility

-

solubility

-

mol wt

24.5 kDa

mol wt

23-30 kDa

mol wt

37 kDa

UniProt accession no.

P07858

UniProt accession no.

P08311

UniProt accession no.

-

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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