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C1639

Collagenase from Clostridium histolyticum

0.2 μm filtered, for general use, Type I-S, 0.2-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid

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Synonym(s):

Clostridiopeptidase A

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Size/SKUAvailabilityPrice
50 mg

Estimated to ship onNovember 16, 2026fromMILWAUKEE

$115.00

About This Item

CAS Number:
9001-12-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-582-9
MDL number:
MFCD00130830
EC Number:
3.4.24.3 (BRENDA, IUBMB)
Specific activity:
≥125 CDU/mg solid, 0.2-1.0 FALGPA units/mg solid

$115.00

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Estimated to ship onNovember 16, 2026Details

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Quality Segment

sterility

0.2 μm filtered

form

lyophilized powder

specific activity

≥125 CDU/mg solid, 0.2-1.0 FALGPA units/mg solid

mol wt

68-130 kDa

storage temp.

−20°C

Application

This product is suitable for the disaggregation of human tumor, mouse kidney, human adult and fetal brain, lung and many other epithelia tissues. It has also been shown to be effective in liver and kidney perfusion studies, digestion of pancreas, isolation of nonparenchymal rat liver cells and hepatocyte preparation. Collagenase has also been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products. This enzyme has been tested for the release of heptatocytes at a concentration of approximately 1 mg/mL. Concentrations for digestion range from 0.1 to 5 mg/mL.

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
The collagenase product is a mixture of enzymes secreted by C. histolyticum, with different products differentiated by the relative ratios of the 10-18 components found in the secreted enzymes. The main components are two collagenases, clostripain, and a neutral protease. The synergistic action of these enzymes degrade collagen and other intracellular materialThe action of both collagenase enzymes and the neutral protease is necessary for effective release of cells from tissue. Various types of collagen are the natural substrates for collagenase.

Preparation Note

Prepared from Type I (C0130). A stock solution may be prepared by dissolving 0.05-0.1 mg/mL of collagenase in 50 mM TES buffer containing 0.36 mM calcium chloride (TESCA, pH 7.4) at 37 °C.

Other Notes

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Disclaimer

As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL) and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains 100% activity over 7 hours when held on ice.

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This Item
C1889C8176C7926
Collagenase from Clostridium histolyticum 0.2 μm filtered, for general use, Type I-S, 0.2-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid

Sigma-Aldrich

C1639

Collagenase from Clostridium histolyticum

Collagenase from Clostridium histolyticum 0.2 μm filtered, suitable for release of physiologically active rat hepatocytes, Type IV-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

Sigma-Aldrich

C1889

Collagenase from Clostridium histolyticum

Collagenase from Clostridium histolyticum Sigma Blend Type L, ≤1.0 FALGPA units/mg solid

Sigma-Aldrich

C8176

Collagenase from Clostridium histolyticum

Collagenase from Clostridium histolyticum Sigma Blend Type F, ≥2.0 FALGPA units/mg solid

Sigma-Aldrich

C7926

Collagenase from Clostridium histolyticum

description

0.2 μm filtered, for general use, Type I-S, 0.2-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid

description

0.2 μm filtered, suitable for release of physiologically active rat hepatocytes, Type IV-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

description

Sigma Blend Type L, ≤1.0 FALGPA units/mg solid

description

Sigma Blend Type F, ≥2.0 FALGPA units/mg solid

specific activity

0.2-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid

specific activity

≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid

specific activity

≤1.0 FALGPA units/mg solid

specific activity

≥2.0 FALGPA units/mg solid

form

lyophilized powder

form

powder

form

powder

form

powder

sterility

0.2 μm filtered

sterility

0.2 μm filtered

sterility

-

sterility

-

mol wt

68-130 kDa

mol wt

68-130 kDa

mol wt

68-130 kDa

mol wt

68-130 kDa

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000114218
    0000370475
    048M4053V
    0000167339
    0000244440

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