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Collagenase from Clostridium histolyticum

Name: Collagenase from <I>Clostridium histolyticum</I>
Brand: SIGMA
Price: 102 EUR

0.2 μm filtered, Type V-S, ≥1 FALGPA units/mg solid, ≥125 CDU/mg solid

Synonym(s):

Clostridiopeptidase A

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Pack Size	SKU	Availability	Price
50 mg		Check Cart for Availability	€ 102,00

About This Item

CAS Number:

9001-12-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-582-9

MDL number:

MFCD00130830

EC Number:

3.4.24.3 (BRENDA, IUBMB)

Specific activity:

≥1 FALGPA units/mg solid, ≥125 CDU/mg solid

€ 102,00

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Properties

Quality Level

200

sterility

0.2 μm filtered

form

lyophilized powder

specific activity

≥1 FALGPA units/mg solid, ≥125 CDU/mg solid

mol wt

68-125 kDa

storage temp.

−20°C

Description

Application

Collagenase has been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products (AGE). The enzyme has also been used along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues. It is also effective in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes.

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca²⁺) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa (pH optimum: 6.3-8.8). The enzyme recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X is most often a neutral amino acid.

Preparation Note

Prepared from Type V (C9263)

Analysis Note

Also contains clostripain, nonspecific neutral protease, and tryptic activities.

Other Notes

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

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Show Differences

1 of 1

This Item	C1639	C5894	C9263
Sigma-Aldrich C2014 Collagenase from Clostridium histolyticum	Sigma-Aldrich C1639 Collagenase from Clostridium histolyticum	Sigma-Aldrich C5894 Collagenase from Clostridium histolyticum	Sigma-Aldrich C9263 Collagenase from Clostridium histolyticum
specific activity ≥1 FALGPA units/mg solid, ≥125 CDU/mg solid	specific activity ≥125 CDU/mg solid, 0.2-1.0 FALGPA units/mg solid	specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid	specific activity ≥1 FALGPA units/mg solid, >125 CDU/mg solid
form lyophilized powder	form lyophilized powder	form lyophilized powder	form powder
sterility 0.2 μm filtered	sterility 0.2 μm filtered	sterility non-sterile; 0.2 μm filtered	sterility -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt 68-125 kDa	mol wt 68-130 kDa	mol wt 68-130 kDa	mol wt 113-114 kDa
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

0000503750

0000302834

0000137861

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Protocols

Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

Global Trade Item Number

SKU	GTIN
C2014-50MG	04061833474990

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