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C2181

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Cy3 antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Cy3 Anti-Mouse IgG, Cy3 Mouse IgG, Sheep Anti-Mouse IgG

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Pack SizeSKUAvailabilityPrice
1 mL
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$396.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD02179333
Conjugate:
CY3 conjugate
Clone:
polyclonal
Application:
IHC (p)
Citations:
121

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biological source

sheep

Quality Level

200

conjugate

CY3 conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant protein in human serum. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4.. The IgG heavy chain region is mapped to human chromosome 14. Igs have two heavy (H) and two light (L) chains, held together by disulfide linkages. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The L chain comprises of one variable N-terminal region and a constant C-terminal region.

Application

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-Cy3 antibody produced in sheep was used for BrdU staining of frozen quail muscle cross sections at a dilution of 1:200 to analyze activated, proliferating muscle precursor cells.
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment−Cy3 antibody produced in sheep has been used:
  • in immunolabeling of Hela cells
  • as secondary antibody in immunocytochemistry of dendritic cells
  • as secondary antibody in immunofluorescence analysis of mesenchymal stem cells
  • as secondary antibody in immunofluorescence staining keratinocyte cell lines
  • in immunohistochemistry of articular cartilage

The product binds to all mouse Igs and is useful when trying to avoid background staining due to the presence of Fc receptors.

Biochem/physiol Actions

Digestion of IgG by papain results in the generation of fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallizable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of Cy3 to Anti-Mouse IgG (whole molecule) F(ab′)2 fragment antibody allows for the visualization of protein by fluorescent microscopy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with human samples.

Other Notes

Antibody adsorbed with human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
A3563C2306A2179
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Cy3 antibody produced in sheep affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

C2181

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Cy3 antibody produced in sheep

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Alkaline Phosphatase antibody produced in sheep affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3563

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Alkaline Phosphatase antibody produced in sheep

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–Cy3 antibody produced in sheep affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

C2306

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment–Cy3 antibody produced in sheep

Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A2179

Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

CY3 conjugate

conjugate

alkaline phosphatase conjugate

conjugate

CY3 conjugate

conjugate

alkaline phosphatase conjugate

biological source

sheep

biological source

sheep

biological source

sheep

biological source

goat

species reactivity

mouse

species reactivity

mouse

species reactivity

rabbit

species reactivity

mouse

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25, western blot (chemiluminescent): 1:80,000

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous solution

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000542430
0000542430
0000360599
0000310611
0000310611

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Global Trade Item Number

SKUGTIN
C2181-1ML04061833475317

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