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Collagenase from Clostridium histolyticum

Name: Collagenase from <I>Clostridium histolyticum</I>
Brand: SIGMA
Price: 384 CAD

0.2 μm filtered, high purity, purified by chromatography, Type VII-S, ≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)

Synonym(s):

Clostridiopeptidase A

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Pack Size	Availability	Price
1500 units	Please contact Customer Service for Availability	$384.00
3000 units	Please contact Customer Service for Availability	$537.00
15000 units	Please contact Customer Service for Availability	$1,830.00 $1,555.50

About This Item

CAS Number:

9001-12-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-582-9

MDL number:

MFCD00130830

EC Number:

3.4.24.3 (BRENDA, IUBMB)

Specific activity:

≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)

$384.00

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Properties

Quality Level

200

sterility

0.2 μm filtered

form

lyophilized powder

specific activity

≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)

purified by

chromatography

solubility

TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C

storage temp.

−20°C

Description

Application

Collagenase has been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products (AGE). The enzyme has also been used along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues. It is also effective in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes.

Collagenase from Clostridium histolyticum has been used:

to induce intracerebral hemorrhage (ICH) in mice
to digest collagen 3D gels for immunofluorescence
to cause focal blood extravasation and hemorrhage in mouse

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca²⁺) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa (pH optimum: 6.3-8.8). The enzyme recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X is most often a neutral amino acid.

Clostridium histolyticum contains two classes of collagenases. Clostridial collagenases are more effective than mammalian collagenases. It cleaves at multiple cleavage sites within the collagen triple helix. Clostridial collagenases are involved in bacterial invasion in gas gangrene.

Preparation Note

Prepared from Type VII (C0773). A stock solution may be prepared by dissolving 0.05-0.1 mg of collagenase in one mL of 50 mM TES buffer containing 0.36 mM calcium chloride (TESCA), pH 7.4 at 37 °C.

Other Notes

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

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Show Differences

1 of 1

This Item	C0255	C2799	C2014
Sigma-Aldrich C2399 Collagenase from Clostridium histolyticum	Sigma-Aldrich C0255 Collagenase from Clostridium histolyticum	Sigma-Aldrich C2799 Collagenase from Clostridium histolyticum	Sigma-Aldrich C2014 Collagenase from Clostridium histolyticum
specific activity ≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)	specific activity ≥500 CDU/mg solid (CDU = collagen digestion units)	specific activity ≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)	specific activity ≥1 FALGPA units/mg solid, ≥125 CDU/mg solid
form lyophilized powder	form lyophilized powder	form powder	form lyophilized powder
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
solubility TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C	solubility TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C	solubility -	solubility -
sterility 0.2 μm filtered	sterility -	sterility -	sterility 0.2 μm filtered
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Protocols

Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

A Role of Complement in the Pathogenic Sequelae of Mouse Neonatal Germinal Matrix Hemorrhage.

Mohammed Alshareef et al.

International journal of molecular sciences, 23(6) (2022-03-26)

Germinal matrix hemorrhage (GMH) is a devastating disease of infancy that results in intraventricular hemorrhage, post-hemorrhagic hydrocephalus (PHH), periventricular leukomalacia, and neurocognitive deficits. There are no curative treatments and limited surgical options. We developed and characterized a mouse model of

Voxel-Based Analysis of [18F]-FDG Brain PET in Rats Using Data-Driven Normalization.

Silke Proesmans et al.

Frontiers in medicine, 8, 744157-744157 (2021-11-09)

Introduction: [18F]-FDG PET is a widely used imaging modality that visualizes cellular glucose uptake and provides functional information on the metabolic state of different tissues in vivo. Various quantification methods can be used to evaluate glucose metabolism in the brain

Transient receptor potential vanilloid 4 agonist GSK1016790A improves neurological outcomes after intracerebral hemorrhage in mice.

Yasunori Asao et al.

Biochemical and biophysical research communications, 529(3), 590-595 (2020-08-02)

Intracerebral hemorrhage (ICH) is one of the most severe subtypes of stroke with high morbidity and mortality. Although a lot of drug discovery studies have been conducted, the drugs with satisfactory therapeutic effects for motor paralysis after ICH have yet

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Global Trade Item Number

SKU	GTIN
C2399-15KU	04061832699967
C2399-7.5KU	04061832699981
C2399-1.5KU	04061832699950
C2399-3KU	04061832699974

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