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Collagenase from Clostridium histolyticum

Name: Collagenase from <I>Clostridium histolyticum</I>
Brand: SIGMA
Price: 436 USD
Availability: InStock

powder, suitable for cell culture, ≥4 FALGPA units/mg solid, high purity, ≥700 CDU/mg solid (CDU = collagen digestion units)

Synonym(s):

Clostridiopeptidase A

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Size/SKU	Availability	Price
7500 units	Available to ship TODAYfromMILWAUKEE	$436.00
15000 units	Check Cart for Availability	$770.00

About This Item

CAS Number:

9001-12-1

UNSPSC Code:

12352204

NACRES:

NA.75

EC Number:

232-582-9

MDL number:

MFCD00130830

EC Number:

3.4.24.3 (BRENDA, IUBMB)

Specific activity:

≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)

Biological source:

Clostridium histolyticum

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Properties

biological source

Clostridium histolyticum

Quality Segment

200

form

powder

specific activity

≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)

mol wt

68-130 kDa

purified by

chromatography

technique(s)

cell culture | mammalian: suitable

pH

7.4

foreign activity

neutral protease and clostripain ≤1 unit/mg solid

shipped in

dry ice

storage temp.

−20°C

Description

Application

This product is suitable for the disaggregation of human tumor, mouse kidney, human adult and fetal brain, lung and many other epithelia tissues. It has also been shown to be effective in liver and kidney perfusion studies, digestion of pancreas, isolation of nonparenchymal rat liver cells and hepatocyte preparation. Collagenase has also been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products. This enzyme has been tested for the release of heptatocytes at a concentration of approximately 1 mg/mL. Concentrations for digestion range from 0.1 to 5 mg/mL.

Use for the digestion of collagen and the release of adherent cells from substrates.

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

The collagenase product is a mixture of enzymes secreted by C. histolyticum, with different products differentiated by the relative ratios of the 10-18 components found in the secreted enzymes. The main components are two collagenases, clostripain, and a neutral protease. The synergistic action of these enzymes degrade collagen and other intracellular material. The action of both collagenase enzymes and the neutral protease is necessary for effective release of cells from tissue. Various types of collagen are the natural substrates for collagenase.

Preparation Note

This collagenase is obtained from the culture filtrate of type VII (C0773) Clostridium histolyticum. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. Solutions are typically prepared at 1-2 mg/mL in TESCA buffer (containing 50 mM TES, 0.36 mM Calcium chloride, pH 7.4 at 37°C.

Other Notes

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Disclaimer

As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL) and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains 100% activity over 7 hours when held on ice.

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This Item	C0773	C2674	C2399
Sigma-Aldrich C2799 Collagenase from Clostridium histolyticum	Sigma-Aldrich C0773 Collagenase from Clostridium histolyticum	Sigma-Aldrich C2674 Collagenase from Clostridium histolyticum	Sigma-Aldrich C2399 Collagenase from Clostridium histolyticum
description powder, suitable for cell culture, ≥4 FALGPA units/mg solid, high purity, ≥700 CDU/mg solid (CDU = collagen digestion units)	description high purity, purified by chromatography, Type VII, ≥4 FALGPA units/mg solid, lyophilized powder, ≥700 CDU/mg solid (CDU = collagen digestion units)	description lyophilized powder, ≥125 CDU/mg solid (CDU = collagen digestion units), 0.5-5.0 FALGPA units/mg solid	description 0.2 μm filtered, high purity, purified by chromatography, Type VII-S, ≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)
specific activity ≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)	specific activity ≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)	specific activity ≥125 CDU/mg solid (CDU = collagen digestion units), 0.5-5.0 FALGPA units/mg solid	specific activity ≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)
biological source Clostridium histolyticum	biological source -	biological source Clostridium histolyticum	biological source -
technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) cell culture \| mammalian: suitable, single cell analysis: suitable	technique(s) -
form powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt 68-130 kDa	mol wt 68-130 kDa	mol wt 68-130 kDa	mol wt -

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c