C3515
Catalase from Aspergillus niger
ammonium sulfate suspension, ≥4,000 units/mg protein
Synonym(s):
H2O2:H2O2 oxidoreductase
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 mg | Check Cart for Availability | $128.00 | |
| 25 mg | Check Cart for Availability | $210.00 | |
| 100 mg | Check Cart for Availability | $637.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
MDL number:
Specific activity:
≥4,000 units/mg protein
Biological source:
Aspergillus niger
$128.00
Check Cart for Availability
Diagnostics Manufacturers should use alternative grade product: SRE0041
biological source
Aspergillus niger
Quality Level
form
ammonium sulfate suspension
specific activity
≥4,000 units/mg protein
mol wt
tetramer ~250 kDa
storage condition
(Tightly closed)
technique(s)
FISH: suitable
shipped in
wet ice
storage temp.
2-8°C
SMILES string
O(CC)C(=O)c1ccc(cc1)O
InChI
1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
InChI key
NUVBSKCKDOMJSU-UHFFFAOYSA-N
General description
Application
Catalase from Aspergillus niger has been used:
- as a positive control during the functional characterization of Clostridium difficile spore coat proteins.[3]
- as a component of the catalase solution to prepare GLOX buffer with enzymes to maintain the embryos of Caenorhabditis elegans before single-molecule fluorescence in situ hybridization (smFISH) studies[4]
- as a component of the imaging buffer for stochastic optical reconstruction microscopy (STORM) imaging of platelet-rich plasma[5]
- as a supplement in Todd Hewitt media plus 0.5 % yeast extract (THY) media for the neutralization of pneumococcal H2O2[6]
Biochem/physiol Actions
Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Isoelectric point is found to be 6.5. Optimum pH for catalytic activity is 7.0. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.
Catalase is involved in catalyzing the degradation of hydrogen peroxide (H2O2) to water and free oxygen. Catalase is used commercially for decomposing H2O2 as it is added as an antimicrobial agent in process streams.[7] It also catalyzes the oxidation of electron donors like ethanol and phenols during lower concentrations of H2O2.[8] Catalase shows antioxidant activity by protecting cells from higher levels of reactive oxygen species (ROS). Increased expression of catalase is observed in chronic lymphocytic leukemia, gastric cancer, glioma, and melanoma. Decreased levels of catalase are seen in acute myeloid leukemia, lung, pancreatic, prostate, and skin (non-melanoma) cancers. Hence catalase exhibits a dual role in cancer.[1]
Physical form
Suspension in 3.2 M (NH4)2SO4 solution, pH 6.0
Analysis Note
Protein determined by biuret
Other Notes
One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.
Disclaimer
Freezing of solutions is not recommended.
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) FISH: suitable | technique(s) - | technique(s) GC/MS: suitable | technique(s) - |
| biological source Aspergillus niger | biological source - | biological source bovine liver | biological source - |
| specific activity ≥4,000 units/mg protein | specific activity ≥10,000 units/mg protein, ≥4000 units/mg dry wt. | specific activity 10,000-40,000 units/mg protein | specific activity 40,000-60,000 units/mg protein (E1%/405) |
| form ammonium sulfate suspension | form lyophilized powder | form aqueous suspension | form aqueous suspension |
| mol wt tetramer ~250 kDa | mol wt - | mol wt tetramer ~250 kDa | mol wt tetramer ~250 kDa |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
This procedure may be used for all Catalase products.
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
L Brunelli et al.
Free radical biology & medicine, 30(7), 709-714 (2001-03-29)
Previously, we found that catalase enhanced the protection afforded by superoxide dismutase to Escherichia coli against the simultaneous generation of superoxide and nitric oxide (Brunelli et al., Arch. Biochem. Biophys. 316:327-334, 1995). Hydrogen peroxide itself was not toxic in this
Seleipiri Charles et al.
Analytical chemistry, 93(3), 1369-1376 (2020-12-24)
Recent development in fluorescence-based molecular tools has contributed significantly to developmental studies, including embryogenesis. Many of these tools rely on multiple steps of sample manipulation, so obtaining large sample sizes presents a major challenge as it can be labor-intensive and
J Fiedurek et al.
Journal of applied microbiology, 89(1), 85-89 (2000-08-17)
A novel method for increasing dissolved oxygen concentration in culture media has been developed. It involves adding hydrogen peroxide (H2O2) to the medium which is then decomposed to oxygen and water by catalase. Some factors affecting oxygenation of culture were
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C3515-10MG | 04061833485941 |
| C3515-25MG | 04061833485958 |
| C3515-100MG | 04061832729107 |