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Ciliary Neurotrophic Factor human

Name: Ciliary Neurotrophic Factor human
Brand: SIGMA
Price: 296 USD
Availability: InStock

98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

CNTF

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Size/SKU	Availability	Price
10 μg	Check Cart for Availability	$296.00
20 μg	Check Cart for Availability	$420.00

About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00676720

Biological source:

human

Recombinant:

expressed in E. coli

Assay:

≥98% (SDS-PAGE and HPLC)

Form:

lyophilized powder

Mol wt:

22.8 kDa

Impurities:

<1 EU/μg

$296.00

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Properties

Product Name

Ciliary Neurotrophic Factor human, CNTF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biological source

human

Quality Segment

200

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE and HPLC)

form

lyophilized powder

potency

50-150 ng/mL ED₅₀

quality

endotoxin tested

mol wt

22.8 kDa

packaging

pkg of 10 and 20 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<1 EU/μg

UniProt accession no.

P26441

storage temp.

−20°C

Description

General description

The CNTF (ciliary neurotrophic factor) gene encodes a cytokine, and is mapped to human chromosome 11q12.1.

Application

Ciliary Neurotrophic Factor human has been used to study its effect on photoreceptor neuroprotection and Muller glial cell proliferation in zebrafish retina. It has also been used to study the effects of intravitreal injection of ciliary neurotrophic factor (CNTF) on photoreceptor injury and BRB (blood-retinal barrier) breakdown in transgenic model.

Biochem/physiol Actions

Ciliary neurotrophic factor (CNTF) was first identified as a survival factor for neurons from the ciliary ganglion of chicken embryos. Most of its known actions are restricted to cells of the nervous system, including motor neurons, sympathetic ganglion neurons, sensory neurons, hippocampal neurons, and medial septal neurons. CNTF also prevents degeneration of motor axons after axotomy and promotes astrocyte differentiation and oligodendrocyte survival and maturation. Outside the nervous system, CNTF maintains embryonic stem cells in an undifferentiated, pluripotent state. CNTF is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). CNTF exerts its actions through the activation of the high-affinity CTNF receptor complex, which contains the ligand-binding α-subunit (CNTF Rα) and two signal transducing β-subunits (LIF Rβ and gp130). The LIF Rβ subunit is also shared by receptors for LIF and OSM. The gp130 subunit is also shared by receptors for LIF, OSM, IL-6, and IL-11. CNTF is localized in the cell nucleus subsequent to receptor binding. Human and rat CNTF share ~83% sequence homology and show cross-reactivity in bioactivity.

Ciliary neurotrophic factor was first identified as a survival factor for neurons from the ciliary ganglion of chicken embryos. Most of its known actions are restricted to cells of the nervous system, including motor neurons, sympathetic ganglion neurons, sensory neurons, hippocampal neurons and medial septal neurons. CNTF also prevents degeneration of motor axons after axotomy and promotes astrocyte differentiation and oligodendrocyte survival and maturation.

Physical form

Lyophilized containing 5mM Sodium Phosphate, pH 7.5, 75mM NaCl

Analysis Note

The proliferative activity is tested in a cell proliferation assay using the cytokine-dependent human erythroleukemic cell line, TF-1.

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This Item	G1777	G1401	MAB338
Sigma-Aldrich C3710 Ciliary Neurotrophic Factor human	Sigma-Aldrich G1777 Glial Cell Line-derived Neurotrophic Factor human	Sigma-Aldrich G1401 Glial Cell Line-derived Neurotrophic Factor from rat	Sigma-Aldrich MAB338 Anti-Ciliary Neurotrophic Factor Antibody, clone 4-68
description CNTF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture	description recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture	description recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)	description clone 4-68, Chemicon^®, from mouse
assay ≥98% (SDS-PAGE and HPLC)	assay -	assay ≥97% (SDS-PAGE)	assay -
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in baculovirus infected Sf21 cells	recombinant -
biological source human	biological source human	biological source rat	biological source mouse
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) western blot: suitable, immunohistochemistry: suitable
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
form lyophilized powder	form lyophilized powder	form lyophilized powder	form -

Storage Class

13 - Non Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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