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Anti-Corticotropin Releasing Factor antibody produced in rabbit

Name: Anti-Corticotropin Releasing Factor antibody produced in rabbit
Brand: SIGMA
Price: 819 CAD

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

CRF Antibody - Anti-Corticotropin Releasing Factor antibody produced in rabbit, Crf Antibody, Anti-CRF

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Pack Size	SKU	Availability	Price
0.1 mL		Please contact Customer Service for Availability	$819.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02096145

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IHC (p), RIA

Citations:

$819.00

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Properties

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

frog, canine, sheep, rat, human, mouse, pig, bovine

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using microwave-treated sections of human placental tissue, microarray: suitable, radioimmunoassay: 1:10,000 using 2-¹²⁵I-His¹²-CRF, human

UniProt accession no.

P06850

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CRH(1392)
mouse ... Crh(12918)
rat ... Crh(81648)

Description

General description

Corticotropin Releasing Factor (CRF), a 41 amino acid neuropeptide, belongs to a family of structurally related peptides including urocortin, sauvagine and urotensin I. CRF is derived from a larger 196 amino acids precursor protein (preproCRF). CRF is widely distributed in the brain and peripheral nervous system, with the highest levels in the hypothalamic paraventricular nucleus (PVN).

Immunogen

synthetic peptide corresponding to the C-terminal of human CRF (amino acids 25-41 with N-terminally added lysine) conjugated to KLH.

Application

Anti-Corticotropin Releasing Factor antibody produced in rabbit has been used in immunohistochemistry[1] (1:2000) and radioimmunoassay.

Biochem/physiol Actions

Corticotropin Releasing Factor (CRF) is critical in the regulation of the hypothalamic-pituitary-adrenal gland axis, and autonomic and behavioral responses to stress. CRF effects include changes in motor and cognitive behavior, appetite control and modulation of the immune system. CRF is a potent stimulator of adrenocorticotropic hormone (ACTH) secretion by corticotroph cells from the anterior pituitary in vitro and in vivo. The effects of CRF are mediated through CRF receptor subtypes CRFR1 and CRFR2 (splice variants). CRF binds with a high affinity to CRF-binding protein (CRF-BP), which inactivates CRF, while ligands that dissociate CRF from CRF-BP exhibit cognition-enhancing properties. CRF overproduction has been implicated in anorexia and depression and may lead to Cushing′s syndrome. CRF levels are correlated with Alzheimer′s Disease (AD) pathogenesis.

The antibody does not cross-react with human or rat CRF (6-33), sheep CRF, rat urocortin, PACAP38, human ACTH, human ACTH (18-39) and sauvagine.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4500466	SAB1405656	SAB4500465
$Anti-Corticotropin Releasing Factor antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich C5348 Anti-Corticotropin Releasing Factor antibody produced in rabbit	Sigma-Aldrich SAB4500466 Anti-CRHR2 antibody produced in rabbit	Sigma-Aldrich SAB1405656 Anti-CRH antibody produced in mouse	Sigma-Aldrich SAB4500465 Anti-CRHR1 antibody produced in rabbit
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 100
antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form affinity isolated antibody
biological source rabbit	biological source rabbit	biological source mouse	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution
technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using microwave-treated sections of human placental tissue, radioimmunoassay: 1:10,000 using 2-¹²⁵I-His¹²-CRF, human, microarray: suitable	technique(s) ELISA: 1:10000, immunofluorescence: 1:100-1:500, western blot: 1:500-1:1000	technique(s) western blot: 1 μg/mL	technique(s) ELISA: 1:5000, immunofluorescence: 1:100-1:500, western blot: 1:500-1:1000

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Differential adaptive responses to chronic stress of maternally stressed male mice offspring.

Sooyoung Chung et al.

Endocrinology, 146(7), 3202-3210 (2005-04-02)

It is well established that stress in early life can alter the activity of the hypothalamus-pituitary-adrenal (HPA) axis, but most studies to date have focused on HPA reactivity in response to a single acute stress. The present study addressed whether

Corticotrophin-releasing factor, related peptides, and receptors in the normal and inflamed gastrointestinal tract.

Roeland Buckinx et al.

Frontiers in neuroscience, 5, 54-54 (2011-05-05)

Corticotrophin-releasing factor (CRF) is mainly known for its role in the stress response in the hypothalamic-pituitary-adrenal axis. However, increasing evidence has revealed that CRF receptor signaling has additional peripheral effects. For instance, activation of CRF receptors in the gastrointestinal tract

Possible pitfalls in the workup of ectopic ACTH secretion illustrated by four rare cases.

Jorianne Boers et al.

BMJ case reports, 12(11) (2019-11-23)

In this case report, we highlight four different cases of ectopic adrenocorticotropic hormone (ACTH) secretion with different pitfalls in the diagnostic workup. Ectopic ACTH secretion (EAS) is an uncommon cause of Cushing's syndrome that accompanies a variety of tumours. It

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Global Trade Item Number

SKU	GTIN
C5348-.1ML	04061833502006

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