The recommended protocol for detection by flow cytometry is provided in the Product Datasheet. This can be found in the More Documents section of the website:
https://www.sigmaaldrich.com/product/sigma/c6680#product-documentation
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/353/139/c6680dat-ms.pdf
C6680
Anti-HNK-1/N-CAM (CD57) antibody, Mouse monoclonal
clone VC1.1, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-HNK-1, Hnk-1 Antibody, Hnk-1 Antibody - Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse, Anti-CD57, Anti-N-CAM
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| Size/SKU | Availability | Price |
|---|---|---|
50 test | Check Cart for Availability | MXP 5,157.00 |
100 test | Check Cart for Availability | MXP 8,884.00 |
About This Item
Conjugate:
unconjugated
Clone:
VC1.1, monoclonal
Application:
FACS, IHC (p)
Citations:
42
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
VC1.1, monoclonal
form
buffered aqueous solution
species reactivity
mouse, rat, feline, human
technique(s)
flow cytometry: 5 μL using 1 × 106 cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype
IgM
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... B3GAT1(27087)
mouse ... B3gat1(76898)
rat ... B3gat1(117108)
General description
CD57/HNK-1 is expressed on a subpopulation of 15-20% of peripheral blood mononuclear cells, about 60% of NK active cells and on a subset of T cells.
Mouse Monoclonal Anti-Human CD57/HNK-1 (mouse IgM isotype) is derived from the VC1.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a homogenate of cat cerebral cortical area 17. Human natural killer-1 (HNK-1) also called β-1,3-glucuronyltransferase (B3GAT1), is mapped to human chromosome 11q25 and belongs to the glucuronyltransferase gene family. CD57/ HNK-1 is expressed on a subpopulation (15-20%) of peripheral blood mononuclear cells. Expression of the antigen was described for about 60% of NK active cells and for a T-cell subset.
Immunogen
A homogenate of cat primary visual cortex (area 17)
Application
Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse has been used in:
- immunohistochemistry[1]
- immunostaining and microscopy
- immunocytochemistry
- immunofluorescence
Biochem/physiol Actions
Human natural killer-1 (HNK-1)/cluster of differentiation (CD57) is also found on some B-cell lines and B lymphocytic leukemia (B-CLL) melanoma lines. The antigen is also detected in lymphoid tissues, certain neuronal subsets, peripheral nerve Schwann cells, and normal, benign, and malignant prostate. Neurofibromas, schwannomas, traumatic neuromassmall cell lung carcinomas frequently express HNK-1. CD57/HNK-1 appears to be expressed in many species ranging from insects to humans. It is thought to be involved in cellular adhesion events and is expressed on several adhesion related proteins including most neural immunoglobulins superfamily molecules.
Recognizes the CD57/HNK-1 human myeloid cell associated surface glycoprotein. The epitope recognized is an N-linked carbohydrate which is present in a variety of glycoproteins and in some glycolipids. It is resistant to formalin fixation and paraffin embedding. VC1.1 antibody and the HNK-1 (Leu7) antibody inhibit the binding of each other. The antibody recognizes myelin associated glycoprotein in some species and a high molecular weight chondroitin sulphate proteoglycan.
5th Workshop: code no. NK 67
5th Workshop: code no. NK 67
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description clone VC1.1, purified from hybridoma cell culture | description clone VC1.1, ascites fluid, buffered aqueous solution | description clone V9, purified from hybridoma cell culture | description clone TH350, purified from hybridoma cell culture |
| clone VC1.1, monoclonal | clone VC1.1, monoclonal | clone V9, monoclonal | clone TH350, monoclonal |
| Gene Information human ... B3GAT1(27087) | Gene Information human ... B3GAT1(27087) | Gene Information human ... VIM(7431) | Gene Information human ... THY1(7070) |
| antibody form purified from hybridoma cell culture | antibody form ascites fluid | antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture |
| species reactivity mouse, rat, feline, human | species reactivity mouse, rat, feline, human | species reactivity hamster, monkey, dog, horse, bovine, rat, gerbil, chicken, pig, feline, human | species reactivity mouse, human, rat |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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how to use the antibody to do flow experiment, can you give me the protocol
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