Collagenase would be soluble in PBS, however it is recommended to dissolve the enzyme in TESCA buffer containing 50 mM TES, 0.36 mM Calcium chloride. Aliquotes may be stored up to 1 year at -20°C. Avoid repeated freeze thaw cycles. Please see the link below to the product information sheet for additional details.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/321/500/c6885pis.pdf
C6885
Collagenase from Clostridium histolyticum
suitable for release of physiologically active rat epididymal adipocytes, Type II, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid
동의어(들):
Clostridiopeptidase A
조직 및 계약 가격을 보려면 를 클릭합니다.
크기 선택
보기 변경
| 팩 크기/SKU | 재고 정보 | 가격 (VAT 별도) |
|---|---|---|
25 mg | 오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea | ₩138,446 |
100 mg | 오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea | ₩228,781 |
500 mg | 오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea | ₩694,565 |
1 g | 오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea | ₩1,170,701 |
5 g | 오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea | ₩4,693,157 |
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-582-9
MDL number:
Specific activity:
≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid
Biological source:
Clostridium histolyticum
biological source
Clostridium histolyticum
Quality Segment
form
powder
specific activity
≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid
suitability
suitable for release of physiologically active rat epididymal adipocytes
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
General description
Clostridium histolyticum is a pathogenic clostridium that produces collagenase. This is a mixture of enzymes containing collagenase, non-specific proteases and clostripain.[1]
Application
Collagenase may be used: [1]
- for the preparation of arterial tissue for the study of advanced glycosylation end products (AGE)
- for use along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues.
- in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes
- for the preparation of viable hepatocytes from rat liver and for the isolation of fat cells from rat adipose tissue
Biochem/physiol Actions
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Collagenase is activated by four gram atom calcium, per mole enzyme. It is inhibited by ethylene glycol-bis(β-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, β-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.Collagenase enzymes and neutral protease plays an important role in the effective release of cells from tissue. Collagenase recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X is most often a neutral amino acid.
Preparation Note
Solutions are typically prepared at 1-2 mg/mL in TESCA buffer (containing 50 mM TES, 0.36 mM Calcium chloride, pH 7.4 at 37°C.
This product also contains clostripain, nonspecific neutral protease, and tryptic activities.
This product also contains clostripain, nonspecific neutral protease, and tryptic activities.
Other Notes
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.
Disclaimer
As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL) and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains 100% activity over 7 hours when held on ice.
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이 품목 | |||
|---|---|---|---|
| biological source Clostridium histolyticum | biological source Clostridium histolyticum | biological source - | biological source Clostridium histolyticum |
| specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid | specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid | specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid | specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid |
| form powder | form powder | form powder | form powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| shipped in wet ice | shipped in - | shipped in - | shipped in wet ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
-
In case I want to make a stock solution of Collagenase type II of C. histolyticum to store at -20°C, can this be made in PBS or should this PBS already be supplemented with CaCl2? This is not specified in the product information sheet. Thank you.
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