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C7926

Collagenase from Clostridium histolyticum

Sigma Blend Type F, ≥2.0 FALGPA units/mg solid

Synonym(s):

Clostridiopeptidase A

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25 mg

Available to ship TODAYfromMILWAUKEE

$83.90
100 mg

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$183.00
500 mg

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$720.00

About This Item

CAS Number:
9001-12-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-582-9
MDL number:
MFCD00130830
EC Number:
3.4.24.3 (BRENDA, IUBMB)
Specific activity:
≥2.0 FALGPA units/mg solid
Biological source:
Clostridium histolyticum

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biological source

Clostridium histolyticum

Quality Segment

form

powder

caseinase activity

≤10 units/mg solid

specific activity

≥2.0 FALGPA units/mg solid

mol wt

68-130 kDa

storage temp.

−20°C

Application

Collagenase from Clostridium histolyticum has been used in determining the degradation rate of collagen biomaterials. It may also be used to determine the enzymatic degradation and enzymatic resistance by the liberated L-leucine measurements.
The enzyme has been used in the isolation of mast cells from adult mice. This study generated a large number of connective tissue-type mast cells by the culture of murine fetal skin cells. The product has also been used in the in vitro biodegradation study of Bombyx mori silk fibroin fibers and films, by exposing them to the enzyme for varied time periods.
Collagenase from Clostridium histolyticum, or Clostridiopeptidase A, has been used in a study to assess contact dermatitis with clostridiopeptidase A contained in Noruxol ointment. Clostridiopeptidase A has also been used in a study to investigate the early and successful enzymatıc debridement via collagenase application to pinna in a preterm neonate.

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)4; β-mercaptoethanol; glutathione, reduced; thioglycolic acid, sodium; and 2,2′-dipyridyl; 8-hydroxyquinoline are known to inhibit the enzyme activity.

Other Notes

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

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This Item
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Collagenase from Clostridium histolyticum Sigma Blend Type F, ≥2.0 FALGPA units/mg solid

Sigma-Aldrich

C7926

Collagenase from Clostridium histolyticum

Collagenase from Clostridium histolyticum Sigma Blend Type L, ≤1.0 FALGPA units/mg solid

Sigma-Aldrich

C8176

Collagenase from Clostridium histolyticum

Collagenase from Clostridium histolyticum 0.2 μm filtered, for general use, Type I-S, 0.2-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid

Sigma-Aldrich

C1639

Collagenase from Clostridium histolyticum

Collagenase from Clostridium histolyticum 0.2 μm filtered, suitable for release of physiologically active rat hepatocytes, Type IV-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

Sigma-Aldrich

C1889

Collagenase from Clostridium histolyticum

description

Sigma Blend Type F, ≥2.0 FALGPA units/mg solid

description

Sigma Blend Type L, ≤1.0 FALGPA units/mg solid

description

0.2 μm filtered, for general use, Type I-S, 0.2-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid

description

0.2 μm filtered, suitable for release of physiologically active rat hepatocytes, Type IV-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

specific activity

≥2.0 FALGPA units/mg solid

specific activity

≤1.0 FALGPA units/mg solid

specific activity

0.2-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid

specific activity

0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

biological source

Clostridium histolyticum

biological source

-

biological source

-

biological source

-

form

powder

form

powder

form

lyophilized powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

68-130 kDa

mol wt

68-130 kDa

mol wt

68-130 kDa

mol wt

68-130 kDa

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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