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C9722

Colagenasa from Clostridium histolyticum

Name: Colagenasa from <I>Clostridium histolyticum</I>
Brand: SIGMA
Price: 276 USD

lyophilized powder (from 0.2μm filtered solution), 0.5-5.0 FALGPA units/mg solid, suitable for cell culture

Sinónimos:

Clostridiopeptidasa A

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A ustedes/SKU	Disponibilidad	Precio
50 mg	Comprobar disponibilidad del carrito	US$ 276,00

Acerca de este artículo

Número CAS:

9001-12-1

UNSPSC Code:

12352204

NACRES:

NA.75

EC Number:

232-582-9

MDL number:

MFCD00130830

Número CE:

3.4.24.3 (BRENDA, IUBMB)

Specific activity:

0.5-5.0 FALGPA units/mg solid

Biological source:

Clostridium histolyticum

Concentration:

1 mg/mL

US$ 276,00

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Propiedades

biological source

Clostridium histolyticum

Quality Segment

200

sterility

0.2 μm filtered

form

lyophilized powder (from 0.2μm filtered solution)

specific activity

0.5-5.0 FALGPA units/mg solid

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

pH

7.4

shipped in

ambient

storage temp.

−20°C

Descripción

Application

This product is suitable for the disaggregation of human tumor, mouse kidney, human adult and fetal brain, lung and many other epithelia tissues. It has also been shown to be effective in liver and kidney perfusion studies, digestion of pancreas, isolation of nonparenchymal rat liver cells and hepatocyte preparation. Collagenase has also been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products. This enzyme has been tested for the release of heptatocytes at a concentration of approximately 1 mg/mL. Concentrations for digestion range from 0.1 to 5 mg/mL.

Suitable for use in preparation of single cell suspension for sequencing.

Biochem/physiol Actions

La colagenasa es activada por cuatro átomo-gramos de calcio por mol de enzima. Es inhibida por el ácido etilenglicol-bis(beta-aminoetil éter) - N, N, N′,N′-tetraacético, el beta-mercaptoetanol, el glutatión, el ácido tioglicólico y la 8-hidroxiquinolina.

The collagenase product is a mixture of enzymes secreted by C. histolyticum, with different products differentiated by the relative ratios of the 10-18 components found in the secreted enzymes. The main components are two collagenases, clostripain, and a neutral protease. The synergistic action of these enzymes degrade collagen and other intracellular materialThe action of both collagenase enzymes and the neutral protease is necessary for effective release of cells from tissue. Various types of collagen are the natural substrates for collagenase.

Preparation Note

Solutions are typically prepared at 1-2 mg/mL in TESCA buffer (containing 50 mM TES, 0.36 mM Calcium chloride, pH 7.4 at 37°C. This product also contains clostripain, nonspecific neutral protease and tryptic activities.

Other Notes

Una unidad de digestión de colágeno (CDU) libera péptidos del colágeno del tendón de Aquiles bovino que equivalen en la reacción de color de la ninhidrina a 1,0 μmoles de leucina en 5 horas a pH 7,4 y 37 °C en presencia de iones calcio. Una unidad de hidrólisis FALGPA hidroliza 1,0 μmoles de furilacriloil-Leu-Gly-Pro-Ala por minuto a 25°C. Una unidad de proteasa neutra hidroliza la caseína para producir color equivalente a 1,0 μmoles de tirosina por 5 horas a pH 7,5 y 37°C. Una unidad de clostripaína hidroliza 1,0 μmoles de BAEE por minuto a pH 7,6 y 25°C en presencia de DTT.

Disclaimer

As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL) and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains 100% activity over 7 hours when held on ice.

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Este artículo	C2674	C2799	C0255
Sigma-Aldrich C9722 Colagenasa from Clostridium histolyticum	Sigma-Aldrich C2674 Colagenasa from Clostridium histolyticum	Sigma-Aldrich C2799 Colagenasa from Clostridium histolyticum	Sigma-Aldrich C0255 Colagenasa from Clostridium histolyticum
description lyophilized powder (from 0.2μm filtered solution), 0.5-5.0 FALGPA units/mg solid, suitable for cell culture	description lyophilized powder, ≥125 CDU/mg solid (CDU = collagen digestion units), 0.5-5.0 FALGPA units/mg solid	description powder, suitable for cell culture, ≥4 FALGPA units/mg solid, high purity, ≥700 CDU/mg solid (CDU = collagen digestion units)	description purified by chromatography, ≥500 CDU/mg solid (CDU = collagen digestion units), lyophilized powder
technique(s) cell culture \| mammalian: suitable, single cell analysis: suitable	technique(s) cell culture \| mammalian: suitable, single cell analysis: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) -
specific activity 0.5-5.0 FALGPA units/mg solid	specific activity ≥125 CDU/mg solid (CDU = collagen digestion units), 0.5-5.0 FALGPA units/mg solid	specific activity ≥4 FALGPA units/mg solid, ≥700 CDU/mg solid (CDU = collagen digestion units)	specific activity ≥500 CDU/mg solid (CDU = collagen digestion units)
biological source Clostridium histolyticum	biological source Clostridium histolyticum	biological source Clostridium histolyticum	biological source -
form lyophilized powder (from 0.2μm filtered solution)	form lyophilized powder	form powder	form lyophilized powder
concentration 1 mg/mL	concentration 1 mg/mL	concentration -	concentration -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c