This product has historically been synthetically produced. However, it is best to refer to the lot-specific Certificate of Origin, as the method of manufacture and origin of future lots may be subject to change. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under the ‘Certificate of Origin’ tab or click on the link below to request a lot-specific Certificate of Origin:
https://www.sigmaaldrich.com/product/sigma/d0428#product-documentation
D0428
1 kb DNA Ladder
for DNA electrophoresis
Synonym(s):
1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, DNA gel marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 vial | Estimated to ship onMay 04, 2026fromSAINT LOUIS | $427.00 |
About This Item
NACRES:
NA.25
UNSPSC Code:
41105335
$427.00
Estimated to ship onMay 04, 2026Details
General description
Sigma′s 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis, five μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR® Green, and Nancy-520.
Application
1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during gel electrophoresis.[1]
Other Notes
For optimal resolution, the recommended agarose gel concentration is 0.75%.
Sigma′s 1kb Ladder is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Legal Information
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Related Content
Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.
Lidia Fuentes et al.
Journal of inorganic biochemistry, 203, 110875-110875 (2019-11-11)
One mononuclear and another dinuclear Pd(II) complexes bearing a α-N-heterocyclic thiosemicarbazone ligand have been synthesized, fully characterized and studied as biological agents. In both complexes, the palladium center is coordinated to 3,5-diacetyl-1,2,4-triazol bis(N4,N4-dimethylthiosemicarbazone) via three sites (N, N and S).
Luca Cocolin et al.
Applied and environmental microbiology, 70(3), 1347-1355 (2004-03-10)
In this paper we describe the development of a PCR protocol to specifically detect Brettanomyces bruxellensis and B. anomalus. Primers DB90F and DB394R, targeting the D1-D2 loop of the 26S rRNA gene, were able to produce amplicons only when the
W S Choe et al.
Biotechnology progress, 17(6), 1107-1113 (2001-12-12)
The direct chemical extraction of recombinant L1 protein (the major capsid protein of human papillomavirus type 16) from the cytoplasm of E. coli HMS174(DE3) has recently been demonstrated at high cell density (to OD(600) = 160) without the use of
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D0428-1VL | 04061835572458 |
-
Does this product have biological origin? Or is the DNA synthetically formulated?
1 answer-
Helpful?
-
-
how to use this product. in what ratio we have to add dye in this.
1 answer-
This product has been tested successfully at 2 µL, 5µL, and 7 µL., however for general use, a starting volume of 5 µL is recommended. Dilute 5 µL volume of D0428 with 25 µL of gel loading buffer. Product G2526 is used for testing purposes. For best results, a 0.75% gel is recommended.
See the link below to Gel Loading Buffer, G2526:
https://www.sigmaaldrich.com/product/sigma/g2526Helpful?
-