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D219

Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse

clone 20A, ascites fluid, buffered aqueous solution

Synonym(s):

Anti-CACNA2, Anti-CACNL2A, Anti-CCHL2A, Anti-LINC01112, Anti-lncRNA-N3

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100 μL
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RM 4,056.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD01323189
Conjugate:
unconjugated
Clone:
20A, monoclonal
Application:
IHC (f), WB
Citations:
25

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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

20A, monoclonal

form

buffered aqueous solution

mol wt

antigen 143 kDa (reduced), antigen 220 kDa (non-reduced)

species reactivity

rat, mouse, rabbit, guinea pig

technique(s)

immunohistochemistry (frozen sections): 1:500, western blot: 1:500

isotype

IgG2a

UniProt accession no.

O08532

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Cacna2d1(12293)
rat ... Cacna2d1(25399)

General description

Dihydropyridine Receptor (DHPR) is a heteropentamer calcium ion channel and comprises of α1,α2/δ,β and γ subunits. It is localized in the skeletal muscle in the t-tubule membrane. The α2subunit of DHPR is a glycosylated subunit and is anchored on the membrane.

Immunogen

Rabbit dihydropyridine (DHP) receptors, (α2 subunit)

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse has been used in:
  • immunofluorescent histochemistry
  • surface plasmon resonance binding
  • immunohistochemistry (IHC)
  • immunoblotting

Use of this antibody for immunoblot analysis will allow for detection of a 220 kDa band (native conditions) or a 143 kDa band (denaturing conditions).Use of this antibody for immunohistochemistry of muscle tissue will demonstrate double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines are observed.

Biochem/physiol Actions

Dihydropyridine Receptor (DHFR) α1 subunit is the voltage-sensor. DHPR interacts with ryanodine receptor (RyR) and also mediates excitation-contraction (EC) coupling in cardiac and skeletal muscles. Mutation in the DHPR (E1014K) affects its affinity and selectivity towards calcium permeation. Additionally, it also plays a key role in the synaptic transmission.
Reacts specifically with the α2 subunit of the DHP receptor. Strong reactivity is observed with the α2 subunit in rat, mouse, guinea pig, rabbit skeletal and cardiac muscle, and rat brain and lung tissue. Weaker reactivity is observed with rat kidney, liver and spleen tissues. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Other Notes

This mouse monoclonal IgG2a antibody is supplied as a mouse ascites diluted in phosphate buffered saline containing 0.05% sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse clone 20A, ascites fluid, buffered aqueous solution

Sigma-Aldrich

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Sigma-Aldrich

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antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

20A, monoclonal

clone

XVIF9-G10, monoclonal

clone

34C, monoclonal

clone

S8B-1, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

rat, mouse, rabbit, guinea pig

species reactivity

human, rat, monkey, sheep, rabbit, guinea pig, canine

species reactivity

human, rat, rabbit, canine, mink, bovine, frog, fish, primate, chicken, mouse, sheep

species reactivity

mouse, human, rat

Gene Information

mouse ... Cacna2d1(12293)
rat ... Cacna2d1(25399)

Gene Information

human ... ATP1A3(478)
rat ... Atp1a3(24213)

Gene Information

human ... RYR1(6261), RYR2(6262)
mouse ... Ryr1(20190), Ryr2(20191)
rat ... Ryr1(114207), Ryr2(84025)

Gene Information

rat ... Cacnb2(116600)

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000352717
0000341046
0000331209
0000324762
0000319261

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Related Content

Sade Spencer et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(25), 8605-8611 (2014-06-21)
Relapse to cocaine seeking is associated with potentiated excitatory synapses in nucleus accumbens. α2δ-1 is an auxiliary subunit of voltage-gated calcium channels that affects calcium-channel trafficking and kinetics, initiates extracellular signaling cascades, and promotes excitatory synaptogenesis. Previous data demonstrate that
Cardiac L-type calcium channel (Cav1. 2) associates with gamma subunits
Yang L, et al.
Faseb Journal, 25(3), 928-936 (2011)
Laurent Ferron et al.
iScience, 27(6), 109973-109973 (2024-06-03)
N- and P/Q-type voltage-gated Ca2+ channels are critical for synaptic transmission. While their expression is increased in the dorsal root ganglion (DRG) neuron cell bodies during neuropathic pain conditions, less is known about their synaptic remodeling. Here, we combined genetic
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Global Trade Item Number

SKUGTIN
D219-100UL04061833561850

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