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DMEM/F-12

Name: DMEM/F-12
Brand: SIGMA

With ʟ-glutamine, sodium pyruvate and HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Synonym(s):

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217417

Pricing and availability is not currently available.

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Properties

Product Name

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO₃: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM

shipped in

ambient

storage temp.

2-8°C

Description

General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

Hep-2 cells
mouse fibroblast L‐cells
L‐ Wnt family member 3A (Wnt3a) cells
primary buffalo embryonic fibroblast (BEF)

Preparation Note

Formulated to contain 15.6 grams of powder per liter of medium.

Supplement with 1.2 g/L sodium bicarbonate.

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This Item	D8900	D6434	D8437
Sigma-Aldrich D2906 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham	Sigma-Aldrich D8900 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham	Sigma-Aldrich D6434 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham	Sigma-Aldrich D8437 Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form powder	form liquid	form liquid
components NaHCO₃: no L-glutamine: yes phenol red: no sodium pyruvate: 0.055 g/L glucose: 3.15 g/L HEPES: 15 mM	components sodium pyruvate: 0.055 g/L HEPES: 15 mM NaHCO₃: no L-glutamine: yes glucose: 3.15 g/L phenol red: yes	components L-glutamine: no phenol red: no NaHCO₃: yes HEPES: 15 mM sodium pyruvate: 0.055 g/L glucose: 3.15 g/L	components glucose: 3.15 g/L HEPES: yes L-glutamine: yes phenol red: yes sodium pyruvate: 0.055 g/L
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
Quality Level 500	Quality Level 500	Quality Level 400	Quality Level 400

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Storage Class

11 - Combustible Solids

wgk

WGK 1

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Articles

Nutrient Mixture F-12 Ham Formulation

Global Trade Item Number

SKU	GTIN
D2906-50K	04061834178897
D2906-10L	04061833562666
D2906-10X1L	04061835518944