Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

D4154

Plasmid DNA from E. coli RRI

pUC18, buffered aqueous solution

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
10 μg
Check Cart for Availability
$203.00

About This Item

CAS Number:
9007-49-2
UNSPSC Code:
41105600
NACRES:
NA.85
MDL number:
MFCD00166896
eCl@ss:
32160414

$203.00

Check Cart for Availability
​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

Escherichia coli (RRI)

Quality Level

200

grade

Molecular Biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

SMILES string

[P](=O)(OC2C(OC(C2)N)CO[P](=O)(OC3C(OC(C3)N)CO)O)(OCC1OC(CC1O)N)O

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

General description

Plasmid DNA from Escherichia coli RRI confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene. pUC8 and 9 have nine unique sites within the MCS while pUC18 and 19 have thirteen.

Application

Plasmid DNA from E. coli RRI has also been used for in vitro DNA transfection studies.
Plasmid DNA from Escherichia coli RRI has been used in polymerase chain reaction (PCR).

Biochem/physiol Actions

Foreign DNA inserted at the MCS interrupts the β-galactosidase gene and abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium.
These plasmids confer ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene; pUC8 and 9 have nine unique sites within the MCS while pUC18 and 19 have thirteen.
Foreign DNA inserted at the MCS abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium. The orientations of the MCS regions in the pUC plasmids are analogous to those of the corresponding M13 phage.

Other Notes

pUC18 Plasmid DNA, 10 μg is supplied at approximately 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
D3404D3035262012
Plasmid DNA from E. coli RRI pUC18, buffered aqueous solution

Sigma-Aldrich

D4154

Plasmid DNA from E. coli RRI

Plasmid DNA from E. coli RRI pUC19, buffered aqueous solution

Sigma-Aldrich

D3404

Plasmid DNA from E. coli RRI

Deoxyribonucleic acid from human placenta buffered aqueous solution, sexed, female

Sigma-Aldrich

D3035

Deoxyribonucleic acid from human placenta

Deoxyribonucleic Acid, Sodium Salt, Salmon Testes Purified DNA from salmon testes. Absorption (0.001% solution in phosphate buffer, pH 7.0, 260 nm): ≥0.155.

Millipore

262012

Deoxyribonucleic Acid, Sodium Salt, Salmon Testes

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

solid

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

ambient

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

biological source

Escherichia coli (RRI)

biological source

-

biological source

human placenta

biological source

-

Still not finding the right product?

Explore all of our products under Plasmid DNA from E. coli RRI

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000458561
0000327414
0000327414
SLCB5238
SLBZ8650

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

Nucleoside phosphocholine amphiphile for in vitro DNA transfection
Moreau L, et al.
Molecular Biosystems, 1(3), 260-264 (2005)
Quantification of beta-human papillomavirus DNA by real-time PCR.
Weissenborn SJ
Nature Protocols, 5(1), 1-13 (2010)
Asife Sahinarslan et al.
Acta cardiologica, 68(3), 291-297 (2013-07-26)
Both computed tomography (CTA) and conventional angiography (CCA) can provide direct visualization of the coronary arteries. The aim of the present study was to compare the radiation exposure between CTA and CCA and to search whether this amount of radiation
View All Related Papers

Global Trade Item Number

SKUGTIN
D4154-10UG04061833564103

Questions

1–2 of 2 Questions  

  1. What is the sequence of plasmid pUC18 (product number D4154) and the number of copies of the plasmid contained in this product? It will be used as an internal control for PCR amplification.

    What is the sequence of plasmid pUC18 (product number D4154) and the number of copies of the plasmid contained in this product? It will be used as an internal control for PCR amplification.

    1 answer

    1. The GenBank accession number for the sequence of the high-copy number plasmid pUC18 is VB0025.

      The following link contains the sequence for this plasmid: https://www.addgene.org/50004/sequences/#depositor-full.

      The plasmid is 2,686 base pairs in length and the molecular weight is approximately 1.75 × 10⁶ daltons, so the 10µg should be 3.4 x 10^12 copies.

      Helpful?

  2. user manual for Puc18?

    1 answer

    1. The Puc18 is not supplied with a user manual or specific protocol. Please see the link below to the product data sheet which provides additional information on this plasmid: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/263/858/d4154dat.pdf

      Helpful?

Reviews

No rating value

Active Filters