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D4522

Deoxyribonucleic acid from calf thymus

Type XV, Activated, lyophilized powder

Synonym(s):

Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid

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1 mg

Available to ship TODAYfromMILWAUKEE

$51.40
5 mg
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$190.00
5 x 5 mg
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$697.00

About This Item

CAS Number:
91080-16-9
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
293-507-3
NACRES:
NA.52
MDL number:
MFCD00151698
Biological source:
bovine pancreas
Form:
lyophilized powder
Solubility:
water: slightly soluble
Storage temp.:
−20°C

$51.40

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biological source

bovine pancreas

Quality Level

200

grade

Molecular Biology

type

Type XV

form

lyophilized powder

solubility

water: slightly soluble

storage temp.

−20°C

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels[1] and for DNA quantification by fluorescent assay.[2]

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Preparation Note

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
One mg DNA is approx. 20 A260 units.
Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.[3]

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Deoxyribonucleic acid from calf thymus Type XV, Activated, lyophilized powder

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D8899

Deoxyribonucleic acid, single stranded from calf thymus

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Sigma-Aldrich

D4764

Deoxyribonucleic acid from calf thymus

Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA lyophilized powder

Sigma-Aldrich

D8273

Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

-

biological source

bovine pancreas

biological source

bovine (calf) thymus

biological source

bovine (calf) thymus

biological source

bovine (calf) thymus

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

solubility

water: slightly soluble

solubility

-

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000425610
0000425610
0000402444
0000402444
0000402449

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Claudia Gomes et al.
Life science alliance, 4(11) (2021-09-11)
High levels of autoimmune antibodies are observed in COVID-19 patients but their specific contribution to disease severity and clinical manifestations remains poorly understood. We performed a retrospective study of 115 COVID-19 hospitalized patients with different degrees of severity to analyze
Juan Antonio Aguilar-Pimentel et al.
Scientific reports, 10(1), 18334-18334 (2020-10-29)
Sex steroids, such as estrogens and androgens, are important regulators of the humoral immune response. Studies in female mice have demonstrated that alteration of circulating estrogen concentration regulates antibody-mediated immunity. As males have normally little endogenous estrogen, we hypothesized that
Parul Natvar Patel et al.
Tissue engineering, 11(9-10), 1498-1505 (2005-11-02)
The ultimate goal of this research is to develop an injectable cell-scaffold system capable of permitting adipogenesis to abrogate soft tissue deficiencies resulting from trauma, tumor resection, and congenital abnormalities. The present work compares the efficacy of photopolymerizable poly(ethylene glycol)
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Global Trade Item Number

SKUGTIN
D4522-5MG04061833565391
D4522-1MG04061833565384
D4522-5X5MG04061833565407

Questions

1–7 of 7 Questions  

  1. Could you please confirm whether this product is suitable to be used as a DNA standard for generating a calibration curve (0–200 ng/µL) in such applications? DNA quantification using the Hoechst 33258 dye in a fluorescence-based assay.

    1 answer

    1. The molecular weight for D4522 is reported to be between 10-15 million Daltons prior to activation (nicking). 15,000 kilodaltons x (1kb/660 kilodaltons) = 22.72 kb (on average). The average number of base pairs is not measured, and therefore, this calculation is an estimate. We do not have a molecular weight measurement after it's been nicked, but it is expected to be a range below 22.72 kB. We do not have comparison data to inform us of how they may respond differently as a standard curve for a fluorescent assay. We would suggest checking to see if the mechanics behind your assay are length dependent or could be influenced by the ends being nicked or not. Products like D4764 that are deemed stable for long periods of time do not have a noted expiration or retest date.

      Helpful?

  2. Could you provide the number of base pairs for the D4522 product? For example, is it around 20kb?

    1 answer

    1. The reported molecular weight is between 10-15 million Daltons before activation (nicking), primarily as double-stranded DNA. A double-stranded DNA of 1 kb in length has a molecular weight of 660 kiloDalton. A size of 15 million daltons is equivalent to 15,000 kilodaltons. When multiplied by (1kb/660 kilodaltons), it equals 22.72 kb on average. It's important to note that the average number of base pairs is not measured, so this calculation provides an estimate.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. What is the molecular weight of Product D4522, Deoxyribonucleic acid?

    1 answer

    1. The molecular weight is reported to be between 10-15 million Daltons prior to activation (nicking).

      Helpful?

  5. What is the solution stability of Product D4522, Deoxyribonucleic acid?

    1 answer

    1. Solutions of deoxyribonucleic acid have been stored successfully for several months at 4°C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent.  At low concentrations (25 μg/mL), DNA tends to absorb onto the surfaces of plastic tubes.  It is not recommended to store deoxyribonucleic acid in alkaline solutions since it tends to degrade at pH greater than 8.0.

      Helpful?

  6. Is BSA (bovine serum albumin) used during the method of preparation for Product D4522, Deoxyribonucleic acid?

    1 answer

    1. During the method of preparation, when deoxyribonucleic acid is nicked, BSA is added to the solution.  This is followed by an extraction method to remove all exogenous protein. We do not, however, claim this deoxyribonucleic acid is 100% clean of all proteins (including BSA).

      Helpful?

  7. Is Product D4522, Deoxyribonucleic acid, single or double stranded?

    1 answer

    1. Even though it is activated or "nicked" DNA, we have found that it is primarily double stranded.

      Helpful?

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