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D4540

Dimethyl sulfoxide

≥99.5% (GC), suitable for plant cell culture

Dimethyl sulfoxide
1 of 2 reviewers took part in a promotion

Synonym(s):

DMSO

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Pack SizeSKUAvailabilityPrice
100 mL

Available to ship TODAYfromMILWAUKEE

$58.50
500 mL

Available to ship TODAYfromMILWAUKEE

$144.00
1 L

Available to ship TODAYfromMILWAUKEE

$222.00

About This Item

Linear Formula:
(CH3)2SO
CAS Number:
67-68-5
Molecular Weight:
78.13
UNSPSC Code:
12191502
NACRES:
NA.71
PubChem Substance ID:
24893881
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
MFCD00002089
Assay:
≥99.5% (GC)
Technique(s):
cell culture | plant: suitable, cryopreservation: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)

$58.50

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Quality Level

200

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

assay

≥99.5% (GC)

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

technique(s)

cell culture | plant: suitable, cryopreservation: suitable

impurities

<0.1% water (Karl Fischer)

color

colorless

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Application

Dimethyl sulfoxide has been used:
  • in the preparation of bisphenol A (BPA)
  • in cell proliferation assay (MTT assay)
  • in chemosensitivity assay
  • as the vehicle control in all experiments like apoptosis assay
  • in ethoxyresorufin-O-deethylation (EROD) assay

Biochem/physiol Actions

DMSO (dimethyl sulfoxide), an organic solvent possesses several pharmaceutical activities such as analgesic, diuretic, bacteriostatic, membrane-penetrant, anti-inflammatory, vasodilatory and cholinesterase inhibitory effects. It is a by-product of the lumber industry and used as an industrial solvent. DMSO also serves as a therapeutic agent to treat interstitial cystitis and arthritis.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Compare Similar Items

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Show Differences

1 of 1

This Item
D26530193407-4872
Dimethyl sulfoxide &#8805;99.5% (GC), suitable for plant cell culture

Sigma-Aldrich

D4540

Dimethyl sulfoxide

Dimethyl sulfoxide Hybri-Max&#8482;, 0.2 &#956;m filtered, BioReagent, suitable for hybridoma, &#8805;99.7%, Ampoule

Sigma-Aldrich

D2653

Dimethyl sulfoxide

Dimethyl sulfoxide for inorganic trace analysis, &#8805;99.99995% (metals basis)

Supelco

01934

Dimethyl sulfoxide

Dimethyl sulfoxide &#8805;99.0%, suitable for absorption spectrum analysis

Sigma-Aldrich

07-4872

Dimethyl sulfoxide

Quality Level

200

Quality Level

300

Quality Level

100

Quality Level

-

assay

≥99.5% (GC)

assay

≥99.7%

assay

≥99.9% (GC), ≥99.99995% (metals basis)

assay

≥99.0%

technique(s)

cell culture | plant: suitable, cryopreservation: suitable

technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

technique(s)

-

technique(s)

UV/Vis spectroscopy: suitable

solubility

H2O: miscible (completely)

solubility

H2O: miscible (completely)

solubility

H2O: miscible (completely)

solubility

-

form

liquid

form

liquid

form

liquid

form

liquid

density

1.10 g/mL (lit.)

density

1.10 g/mL (lit.)

density

1.099-1.101 g/mL at 20 °C, 1.10 g/mL (lit.)

density

1.10 g/mL (lit.)

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Explore all of our products under Dimethyl sulfoxide

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP3629
BCCN6901
SHBT3256
PCCS8446
SHBT1983

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Articles

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Cell Culture Fundamentals: Cryopreservation and Storage of Cell Lines

Overview on liquid nitrogen storage and freezing of cell stocks for cell line cryopreservation purposes. Free ECACC handbook download.

Curcumin promotes apoptosis, increases chemosensitivity, and inhibits nuclear factor kappaB in esophageal adenocarcinoma
Hartojo W, et al.
Translational Oncology, 3(2), 99-108 (2010)
Evaluation of the sub-lethal toxicity of Cu, Pb, bisphenol A and polychlorinated biphenyl to the marine dinoflagellate Cochlodinium polykrikoides
Ebenezer V and Ki J S
Algae, 27(1), 63-63 (2012)
Pharmacotherapy of joint and tendon disease
Equine Sports Medicine and Surgery E-Book: Basic and clinical sciences of the equine athlete, 473-473 (2013)
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Global Trade Item Number

SKUGTIN
D4540-1L04061838259295
D4540-500ML04061838259301
D4540-100ML04061838259288
2 Reviews This action will navigate to reviews.

Questions

1–10 of 11 Questions  

  1. Can I use this product (suitable for plant cell culture) for cyropreservation of mammalian cells? Does cryopreservation of mammalian cells require sterile-filtered DMSO?

    1 answer

    1. D4540 is listed as suitable for cryopreservation.
      However, D2650 is already pre-sterile and has documentation with a protocol for cryopreservation of human and animal cell lines.

      See these links:
      https://www.sigmaaldrich.com/product/sigma/d2650
      https://www.sigmaaldrich.com/product/sigma/d2650#product-documentation

      Look for the Product Information Sheet. Also, see this link for more information:
      https://www.sigmaaldrich.com/products/cell-culture-and-analysis/cell-culture-supplements-and-reagents/cell-freezing-reagents

      Helpful?

  2. Can I autoclave this product?

    1 answer

    1. DMSO is not autoclavable in neat form. It is highly unlikely that microorganisms would survive in this environment. Should sterilization be necessary a PTFE, Polypropylene, or Nylon filter membrane are recommended for sterile-filtration.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. What concentration of DMSO do I use in a cell freezing media?

    1 answer

    1. DMSO is usually used at 1-10%, depending on the cell line.  For the cell freezing media that we offer, it is used at 8.7% concentration.

      Helpful?

  5. Is Dimethyl sulfoxide a sterile product?

    1 answer

    1. No, this product is not considered sterile.  Product Nos. D2560 and D2438 are sterile-filtered.

      Helpful?

  6. What is the molarity of DMSO?

    1 answer

    1. DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

      Helpful?

  7. Where does DMSO come from?

    1 answer

    1. DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

      Helpful?

  8. Does DMSO have any synthetic utility?

    1 answer

    1. DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

      Helpful?

  9. What is the DMSO dissolved in?

    1 answer

    1. DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

      Helpful?

  10. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    1 answer

    1. DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

      Helpful?

1–10 of 11 Questions  

Reviews

1 of 2 reviewers took part in a promotion

Active Filters

  1. San Diego
    • Review 1
    • Votes 4
    5 out of 5 stars.

    Works on mammalian cells

    Good for mammalian cell culture treatments of compound

    Helpful?

  2. 1 Ratings-Only Review