The solution storage and stability of this product have not been established. However, the activated or nicked version of this material, product D4522, notes that Solutions of DNA have been stored successfully for several months at 4 °C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. Please see the link below to review additional information available in the datasheet for the D4522:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/630/619/d4522pis.pdf
D4764
Deoxyribonucleic acid from calf thymus
Genomic, unsheared
Synonym(s):
double-stranded template DNA, Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.25 unit | Available to ship TODAYfromMILWAUKEE | $53.40 | |
| 1 unit | Available to ship TODAYfromMILWAUKEE | $136.00 $115.60 | |
| 5 units | Available to ship TODAYfromMILWAUKEE | $457.00 |
About This Item
CAS Number:
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
293-507-3
NACRES:
NA.52
MDL number:
Biological source:
bovine (calf) thymus
Form:
lyophilized powder
Storage temp.:
2-8°C
$53.40
Available to ship TODAYDetails
biological source
bovine (calf) thymus
Quality Level
grade
Molecular Biology
form
lyophilized powder
mol wt
>20 kb
storage temp.
2-8°C
General description
High quality double-stranded template DNA isolated from the thymus of male and female calves.
Application
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay.[1] It was used as reference to determine the DNA content in cartilage tissue digest samples.[2][3]
Biochem/physiol Actions
42% GC; Tm = 87 °C.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Features and Benefits
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
• High quality template DNA
Preparation Note
Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A260 units per mL. ~20 A260 units per mg DNA.
1 of 1
This Item | |||
|---|---|---|---|
| biological source bovine (calf) thymus | biological source bovine (calf) thymus | biological source bovine pancreas | biological source bovine (calf) thymus |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt >20 kb | mol wt ~50 kb | mol wt - | mol wt - |
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| D4764-1UN | 04061833585641 |
| D4764-5UN | 04061833585658 |
| D4764-.25UN | 04061833253038 |
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What are the storage conditions once the DNA is resuspended? Should I keep the DNA at -20ºC or -80ºC?
1 answer-
Helpful?
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how much is 1 unit (i.e. in ug or mg)?
1 answer-
One unit, based on the A260 value, is approximately 50 µg. The general conversion formula is 20 units per milligram. The 0.25 UN package size will contain approximately 12.5 µg, the 1 jUN package size will contain approximately 50 µg, and the 5UN package size will contain approximately 250 µg.
For additional product information, please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/289/542/d4764dat.pdfHelpful?
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