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D5546

DMEM - low glucose

With sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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Size/SKUAvailabilityPrice
500 mL
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$49.50
1 L
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$92.70

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:

$49.50


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Product Name

Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
phenol red: yes
glucose: low
HEPES: no
sodium pyruvate: yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

General description

Listen to an Audio Overview
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a higher concentration of amino acids, vitamins, and additional supplementary components. The original DMEM formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. This modification has been shown to improve the stability of the medium.

Application

Dulbecco′s Modified Eagle′s Medium - low glucose has been used to culture:

  • human osteosarcoma cells and HeLa cells
  • primary human mesenchymal stromal cells (hBMSCs)
  • human embryonic kidney cell lines

Preparation Note

Supplement with 0.584 g/L glutamine.

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description

With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

description

With 1000 mg/L glucose, L-glutamine and sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

description

With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

sterility

sterile-filtered

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

powder

form

liquid

form

liquid

components

NaHCO3: yes
phenol red: yes
glucose: low
HEPES: no
sodium pyruvate: yes
L-glutamine: no

components

L-glutamine: yes
NaHCO3: no
sodium pyruvate: yes
phenol red: yes
HEPES: no
glucose: low

components

glucose: low
sodium pyruvate: no
phenol red: no
L-glutamine: yes

components

HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient


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Storage Class

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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