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D5546

DMEM - low glucose

With sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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500 mL

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$49.50
1 L
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6 x 500 mL
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24 x 500 mL
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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217342

$49.50

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Product Name

Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
phenol red: yes
glucose: low
HEPES: no
sodium pyruvate: yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

General description

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a higher concentration of amino acids, vitamins, and additional supplementary components. The original DMEM formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. This modification has been shown to improve the stability of the medium. 

Application

Dulbecco′s Modified Eagle′s Medium - low glucose has been used to culture:

  • human osteosarcoma cells and HeLa cells[1]
  • primary human mesenchymal stromal cells (hBMSCs)[2]
  • human embryonic kidney cell lines[3]

Preparation Note

Supplement with 0.584 g/L glutamine.

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This Item
D5921D2429D6046
Dulbecco’s Modified Eagle’s Medium - low glucose With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D5546

Dulbecco′s Modified Eagle′s Medium - low glucose

Dulbecco’s Modified Eagle’s Medium - low glucose With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D5921

Dulbecco′s Modified Eagle′s Medium - low glucose

Dulbecco’s Modified Eagle’s Medium - low glucose 10 ×, With 1000 mg/L glucose (1x), without L-glutamine, sodium bicarbonate, and folic acid, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D2429

Dulbecco′s Modified Eagle′s Medium - low glucose

Dulbecco’s Modified Eagle’s Medium - low glucose With 1000 mg/L glucose, L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D6046

Dulbecco′s Modified Eagle′s Medium - low glucose

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

components

NaHCO3: yes
phenol red: yes
glucose: low
HEPES: no
sodium pyruvate: yes
L-glutamine: no

components

phenol red: no
sodium pyruvate: no
glucose: low
HEPES: no
L-glutamine: no
NaHCO3: yes

components

NaHCO3: no
sodium pyruvate: yes
phenol red: yes
glucose: low
L-glutamine: no
HEPES: no

components

L-glutamine: yes
sodium pyruvate: yes
phenol red: yes
NaHCO3: yes
HEPES: no
glucose: low

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM0549
RNBL7074
RNBL4455
RNBL4194
RNBL3241

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Global Trade Item Number

SKUGTIN
D5546-500ML04061833586440
D5546-6X500ML04061833586464
D5546-6X1L04061833586457
D5546-24X500ML04061833586433
D5546-1L04061833586426

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