We only use this product for our primary cell and cell line cultures.
D5648
DMEM - high glucose
With ʟ-glutamine, without sodium bicarbonate and sodium pyruvate, powder, suitable for cell culture
Dulbecco’s Modified Eagle’s Medium - high glucose
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Synonym(s):
DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 L | Please contact Customer Service for Availability | $65.80 | |
| 10 x 1 L | Please contact Customer Service for Availability | $86.80 | |
| 50 L | Please contact Customer Service for Availability | $228.00 |
About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Product Name
Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
phenol red: yes
L-glutamine: yes
NaHCO3: no
HEPES: no
glucose: high
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
Application
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Dulbecco′s Modified Eagle′s Medium - high glucose has been used as the culture medium for various cell types including the human cervical endocervical epithelial End1/E6E7 cells, human adenocarcinoma epithelial cells (HeLa), human alveolar adenocarcinoma epithelial cells (A549), human osteosarcoma epithelial cells, human diploid cell line , primary hepatocytes and synovial tissue
Preparation Note
Formulated to contain 13.4 grams of powder per liter of medium.
Supplement with 3.7 g/L sodium bicarbonate.
1 of 1
This Item | |||
|---|---|---|---|
| form powder | form powder | form powder | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| components phenol red: yes | components sodium pyruvate: no | components glucose: high | components sodium pyruvate: no |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 500 | Quality Level - | Quality Level 500 | Quality Level 400 |
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Cell culture protocol for passaging and splitting suspension cell lines
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| D5648-10X1L | 04061835518951 |
| D5648-50L | 04061833586549 |
| D5648-1L | 04061837468285 |
| D5648-10L | 04061833586532 |