D5652

Dulbecco′s Phosphate Buffered Saline

Name: Dulbecco′s Phosphate Buffered Saline
Brand: SIGMA
Price: 25.6 USD
Availability: InStock

Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture

Synonym(s):

DPBS

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10 L

$25.60

10 X 1 L

$43.20

50 L

$87.80

$25.60

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About This Item

UNSPSC Code:

12352207

eCl@ss:

32129211

NACRES:

NA.25

MDL number:

MFCD00131855

Key Documents

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Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

pH

7.2-7.8

solubility

water: 9.6 g/L, clear

storage temp.

2-8°C

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Related Categories

Biochemicals

Biological Buffers

Phosphate Buffered Saline (PBS)

Phosphates

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Show Differences

1 of 4

This Item	D1283	D8662	D4031
Sigma-Aldrich D5652 Dulbecco′s Phosphate Buffered Saline	D1283 Dulbecco′s Phosphate Buffered Saline	Sigma-Aldrich D8662 Dulbecco′s Phosphate Buffered Saline	Sigma-Aldrich D4031 Dulbecco′s Phosphate Buffered Saline
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
Quality Level 500	Quality Level 400	Quality Level 400	Quality Level 200
form powder	form liquid	form liquid	form liquid
solubility water: 9.6 g/L, clear	solubility -	solubility -	solubility -
storage temp. 2-8°C	storage temp. -	storage temp. 2-8°C	storage temp. 2-8°C
pH 7.2-7.8	pH -	pH -	pH -

General description

Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture.

Application

D-PBS is used to wash cells during preparation and serial transfer.

Dulbecco′s Phosphate Buffered Saline has been used:

as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloy[1]
to dissolve Angelica gigas powder for microencapsulation
to wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assay[2]
to wash amniotic membrane for cellular analyses[3]

Biochem/physiol Actions

Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.

Preparation Note

Formulated to contain 9.6 grams of powder per liter of medium.

Other Notes

This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Coexpression of nonvisual opsin, retinal G protein-coupled receptor, and visual pigments in human and bovine cone photoreceptors.

Zhaoxia Zhang et al.

Molecular vision, 24, 434-442 (2018-07-24)

Retinal G protein-coupled receptor (RGR) mRNA is transcribed in the outer nuclear layer of human retinas; however, it is not known whether the RGR gene is expressed in the rod or cone photoreceptors. In this study, we investigate broader expression

Rotational diffusion of magnetic nanoparticles in protein solutions.

Ana C Bohórquez et al.

Journal of colloid and interface science, 506, 393-402 (2017-07-28)

The rotational diffusion of polyethylene glycol coated magnetic nanoparticles in serum albumin solutions was investigated in a range spanning 0mgmL

Functional enhancement of ultrafine Angelica gigas powder by spray-drying microencapsulation

Choi KO, et al.

LWT--Food Science and Technology, 101, 161-166 (2019)

Expanding upon the Human Myometrial Stem Cell Hypothesis and the Role of Race, Hormones, Age, and Parity in a Profibroid Environment.

Lauren E Prusinski Fernung et al.

The American journal of pathology, 188(10), 2293-2306 (2018-08-04)

Uterine fibroids (UFs) are clonal, hormonally regulated, benign smooth-muscle myometrial tumors that severely affect female reproductive health, although their unknown etiology limits effective care. UFs occur fourfold more commonly in African American women than in Caucasian women, and African American

Updates on the surface antigens of basophils: CD16 on basophils of patients with respiratory or insect venom allergy and the rejection of CD203c and CD63 externalization decoupling by bisindolylmaleimides.

Petr Heneberg et al.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 49(1), 54-67 (2018-10-06)

CD16 was previously suggested to be a new marker of basophils that is subject to downregulation by FcεRI crosslinking. Certain compounds, including supraoptimal concentrations of the PKC inhibitors, bisindolylmaleimides, decouple the release of granules containing CD203c, CD63 and histamine, and

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Global Trade Item Number

SKU	GTIN
D5652-10L	04061838259318
D5652-200L	04061834170662
D5652-5L	04061837468322
D5652-10X1L	04061835514106
D5652-1L	04061837468315
D5652-2000L	04061837469190
D5652-50L	04061835518968

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Dulbecco′s Phosphate Buffered Saline