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Merck

D6434

DMEM/F-12

With HEPES, sodium bicarbonate and sodium pyruvate, without ʟ-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217417

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Product Name

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
phenol red: no
NaHCO3: yes
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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This Item
D2906D8437D8900
Sigma-Aldrich

Sigma-Aldrich

D6434

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Sigma-Aldrich

Sigma-Aldrich

D2906

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Sigma-Aldrich

Sigma-Aldrich

D8437

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Sigma-Aldrich

Sigma-Aldrich

D8900

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

sterility

-

form

liquid

form

powder

form

liquid

form

powder

components

L-glutamine: no
phenol red: no
NaHCO3: yes
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L

components

NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM

components

glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

  • bovine endometrial epithelial cells[1]
  • cancer stem cells[2]
  • breast cancer cell line[3]

Preparation Note

Supplement with 0.365 g/L L-glutamine

related product

Product No.
Description
Pricing

used together

Product No.
Description
Pricing

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
RNBL3401
RNBK9926
RNBK5266
RNBK0683
RNBJ5744

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